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Effects of MEK Inhibitor on PAK-1 Signaling Induced by Insulin Treatment

This figure compares the phosphorylation levels of PAK-1 and b-cat in cells treated with MEK inhibitor PD98059 before insulin stimulation. Western blotting results and densitometric analysis are presented. The impact on c-Myc, cyclin D1, and ERK levels is also investigated.

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Effects of MEK Inhibitor on PAK-1 Signaling Induced by Insulin Treatment

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  1. Supplementary Figure 1 A. B. C. D. b-cat (Ser675) E.

  2. Figure legend: Both control (GIPZ-Scrambled) and GIPZ-PAK-1 shRNAmir expressing cells were pre-treated with or without the MEK inhibitor PD98059 (PD) for 1h prior to insulin treatment. Amounts of phosphorylated PAK-1 (Thr423), PAK-1, c-Myc, cyclin D1, Ser675 phosphorylated -cat, total -cat, p-ERK, ERK and b-actin (as the loading control) were detected by Western blotting and subject to densitometric analysis (n=3). A representative blot is shown in Fig, 4C.

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