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Alcoholic beverages. David S. Seigler Department of Plant Biology University of Illinois Urbana, Illinois 61801 USA seigler@life.illinois.edu http://www.life.illinois.edu/seigler. Alcoholic beverages from plants - Outline. Importance o Historical - origins o Nutritional

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  1. Alcoholic beverages

  2. David S. Seigler Department of Plant BiologyUniversity of IllinoisUrbana, Illinois 61801 USAseigler@life.illinois.eduhttp://www.life.illinois.edu/seigler

  3. Alcoholic beverages from plants - Outline Importance o Historical - origins o Nutritional o Abuse Wines o Botanical - grapes o Process

  4. Beers o Botanical - cereal grains o Process Distilled beverages o Brandies etc. o Whiskeys

  5. Reading • CHAPTER 14 IN THE TEXT, 332 ff.

  6. Introduction • All alcoholic beverages involve the action of fungi. • Most involve the genus Saccharomyces. These yeasts covert six carbon sugars such as glucose to ethanol and carbon dioxide and live under anaerobic conditions. Yeasts can tolerate fairly high concentrations of alcohol (up to about 14-18%) in the medium.

  7. Alcoholic beverages are known from virtually all cultures. These beverages are major social problems, but also sources of nutrition. • Many plants have been used to prepare alcoholic beverages. Mead is made from fermented honey. • Plants often store nutrition as starch. Yeasts cannot use starch. The starch is broken down by enzymes in the plant into sugars. The sugars are then converted by the yeasts into ethanol and carbon dioxide.

  8. Alcohol is a lipid and moves freely across membranes in the stomach. • Alcohol is broken down in humans, but also affects the neurons and is a non-selective central nervous system depressant.

  9. Wines • Wine is fermented fruit juice. The most important fruit is Vitis vinifera (Vitaceae), but any fruit can be used. • Yeasts occur on the skins of most fruits and if the fruits are mashed, the sugar-containing juices begin to ferment.

  10. Yeast on the outside of grapes B. Lehane, Power of Plants, McGraw Hill. New York. 1977

  11. Winemaking probably began as one of the earliest of human enterprises (8000-3000 B.C.). • The wine grape was domesticated by at least 4000 B.C. Wine was used for Egyptian worship ceremonies. • Wine only became a popular beverage about 2000-1000 B.C. in Greece. • About 600 B.C., wine growing reached France.

  12. Grape motif in an Egyptian tomb B. Lehane, Power of Plants, McGraw Hill. New York. 1977

  13. Wine grapes were introduced early into the United States. • The Spanish introduced grapes into California in the 1700's. • Between 1850 and 1860, the root louse, Phylloxera, was introduced inadvertently from North America into Europe. This insect decimated fields of Vitis vinifera. The problem was solved by making hybrids or grafting European grapes on American grape roots.

  14. Grape flowers and fruits, Vitis vinifera, Vitaceae

  15. Vineyards near Lausanne, Switzerland

  16. Many good quality wines are still made in the same way they have been made for centuries. However, most wines are now made by sophisticated highly controlled processes. • Grapes are crushed (still by foot or with crude presses in many parts of the world). • The juice is then often treated with sulfur dioxide to kill native yeasts. • See diagram p. 343.

  17. Newer style equipment for pressing grapes

  18. Grape pomace after pressing

  19. If white wine is to be made, the juice is put into fermentation tanks and the peels and stems re-pressed. • If red wine is to be made, the skins are added. • Special strains of yeasts are added to the liquid from the grapes. These are often highly guarded trade secrets and proprietary substances. • After 8-10 days, the liquid is drawn off and allowed to ferment for another 20 days to one month. Sediment forms.

  20. Wine fermentation tanks

  21. At this point the wine is drawn off and placed in aging tanks. White wines are usually aged from 1 year to 18 months and red wines as long as 5 years.

  22. Stainless steel aging tanks for wine

  23. Aging of wine in barrels

  24. White wines are generally not too good after 5 years; red wines improve up to 30-40 years. • Wine labelling: see p. 486.

  25. Champagne and sparkling wines are made by putting the wine into a bottle with a little added sugar. The carbon dioxide produced carbonates the wine. The sediment is removed and the the wine maintained under pressure.

  26. Aging of champagne in a New York winery

  27. Apples and pears are used to make cider and perry respectively. • To make fortified wines, ethanol or distilled beverages such as cognac are added. • One group (including port, sherry, and madeira) is made exclusively from grapes, whereas the second group includes aperitif wines and other flavorings (such as Dubonnet and vermouth).

  28. As many as 40 million metric tons of grapes are produced per year. • The major wine making countries are the former USSR, France, Italy, USA, Spain, Germany, Australia, Argentina, Chile and South Africa. • Consumption of wine in France is about 30 gal per person per year and about 2 in the U.S.

  29. Beer, ale and stout • Beer making goes back at least 6000 years. The Sumerians had records about brewing of beer. • Early brewing is intimately linked with bread making. This is one way of making the grains more edible. • Although the Egyptians used wine for religious ceremonies, they commonly drank beer.

  30. Yeasts were developed and saved to give better qualities of beer. Today beer making is also a quite sophisticated process. • The three main ingredients are barley malt, hops and water. • As much as 7.5 X 109 liters of beer are made per year (2 X 109 gallons).

  31. Malt • Malt is sprouted grain that has been dried. • Barley (Hordeum vulgare, Poaceae or Gramineae), is preferred for several reasons. The husks stay on the kernels and add some of the flavor. Also, barley contains higher concentrations of the enzymes needed for converting starches into sugars.

  32. The grain is steeped in water and allowed to sit where it is warm until germination begins. The grain synthesizes hydrolytic enzymes that convert starch to sugar. The cell walls of the endosperm break down. The mixture is then dried at 130 to 200 C.

  33. The brewing process • See diagram p. 493. • The process is now highly mechanized. • The malt is extracted at 68-73 °C for 2-6 hours, a process called mashing. During mashing, the enzymes diffuse into the solution and break down the starch in the malt and the adjuncts. Proteins are degraded into amino acids.

  34. The malted grain is dried, milled, mashed, and strained.Adjuncts are added at this stage. Anheuser Busch Brewing Co., St. Louis

  35. Adjuncts • Adjuncts are unmalted grains that are added. Barley, rice, wheat, or corn grits are often used. • In other countries, potatoes or cassava may be used. • Adjuncts are commonly used in the U.S., but usually not in Europe. They are less expensive than barley malt. Corn and rice are both precooked before being added.

  36. The wort is made by boiling the extracted malt mixture with hops. Anheuser Busch Brewing Co., St. Louis

  37. Hops (Humulus lupulus, Cannabaceae) • Hops (Humulus lupulus, Cannabaceae) were only added to beer after 700 A.D. • The English only started adding them in the 1500's. Sometimes other plants were added for flavoring before that time. Hops not only add flavor but help to coagulate and precipitate proteins. • The female inflorescences are used. They contain a glandular exudate with the flavor properties.

  38. Hops, Humulus lupulus, Cannabaceae, fruits

  39. Hops in cultivation in Kent, England

  40. Hops are then added and the liquid boiled. • This boiling step does several things. • The yeasts present are killed. • Compounds in the hops are converted to bitter tasting compounds.

  41. The resulting liquid, called wort, contains sugars, some starches, proteins and amino acids, among other components. • The spent malt and adjuncts often are used for cattle feed.

  42. In the process of making the wort, enzymes in the mixture are denatured. • Compounds in the hops are converted into the compounds that give beer its bitter taste. The hops are then removed. • The liquid is pumped to fermentation tanks and yeast is added. • Saccharomyces cerevisiae is a top fermenting yeast and S. uvarum is a bottom fermenting yeast. The latter is used to make ales and lager beers.

  43. Fermentation and aging of beer Anheuser Busch Brewing Co., St. Louis

  44. Carbon dioxide is captured and is a major byproduct of brewing. • The mixture is allowed to ferment for 7-12 days at cool temperatures. • The resultant liquid is transferred and allowed to age for 2-3 weeks. • Proteins precipitate out and some other chemical modifications take place.

  45. In the U.S., most of the beers are pasteurized or micropore filtered to remove yeasts. The beer is usually carbonated.

  46. Sake • Sake is made from rice and is a traditional beverage in Japan. It is a fermented grain beverage. • Aspergillus oryzae is used to convert the starch to sugar and then yeast added. Both are involved in the fermentation process. • The mixture is fermented for about 25 days. • The alcohol content is about 18-19%. The beverage is allowed to mature for about 40 days. It is consumed before 1 year.

  47. Pulque • Pulque is made by fermenting the juice of Agave species, especially A. americana. Pulque made from Agave tequilana is distilled to produce tequila.

  48. Maguey, Agave americana, Agavaceae http://commons.wikimedia.org/wiki/Image:Agave_americana.jpg

  49. Collecting the agua miel with an ocote