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A C ompact Video M icroscope I maging S ystem With Intelligent Controls ( CMIS ) S tereo I maging V elocimetry US Patent # 5,905,568 - Stereo imaging velocimetry (1999) US Patent # 6,603,535 - Stereo imaging velocimetry system and method (2003)

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slide1

A Compact Video Microscope Imaging System With Intelligent Controls (CMIS)

StereoImagingVelocimetryUS Patent #5,905,568 - Stereo imaging velocimetry (1999)

US Patent #6,603,535 - Stereo imaging velocimetry system and method (2003)

The World's first 3D full-field quantitative and qualitative analysis tool that is portable and easily adaptable to shuttle experiments, ground-based investigations and industrial applications.

byDr. Bilal Mark McDowell Bomani

NASA Glenn Research Center

slide3

Downlink

Uplink

Communications

Link

slide4

10”x1.5”x1.5”

1/2” color CCD

768x494 pixels

up to 3X mag

11.5”x2”x2”

1/2” color CCD

768x494 pixels

up to 70X mag

12”x24”x12”

Zeiss Conventional Microscope

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Intelligent Algorithms

Instrumentation

and Control Systems

Machine Vision /

Image Processing

Customization!!!

Customization!!!

Hardware and

Software Integration

Web-Enabled

Technologies

Intelligent Graphical

User Interface

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CMIS is a miniature machine vision system, which combines intelligent image processing with remote control capabilities. The software also has a user-friendly interface, which can be used independently of the hardware for further post-experiment analysis.

slide7

Auto-Focus Capabilities

Out-of-Focus Image FFT of Out-of-Focus Image

In-Focus Image FFT of In-Focus Image

slide8

CMISPotential Uses and Applications

  • Microscopy – Interface Detection and Tracking

 Bio-Medical – Cell Labeling & Tracking

  • Bio-Medical – Cell Detection and Feature ExtractionIn-Line Process Inspection – Surface Identification
  • Web-Enabling Technologies – Remote CMIS
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Bio-Medical – Cell Labeling & Tracking

Identify Each Individual Sphere

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Bio-Medical – Cell Labeling & Tracking

Label Each Sphere as Full or Partial

slide15

Bio-Medical – Cell Labeling & Tracking

Determine Sphere Displacements in Original Image

slide16

Bio-Medical – Cell Labeling & Tracking

# Number of noise particles (< 3 pixels):       396# Number of small particles (< 50 pixels):      171# Closest to center: 4

slide25

In-Line Process Inspection – Surface Identification

Ideal Surface Template

Defect Case #1 Defect Case #2

slide26

In-Line Process Inspection – Surface Identification

Ideal Surface Template

Defect Case #1 Defect Case #2

slide30

Year 2000Imaging Solution of the Year!!!from

  • Advanced Imaging Magazine:
  • Medical Imaging, Bioscience, and Scientific Analysis
  • NASA CMIS Research Team:
  • Dr. Mark McDowell
  • Elizabeth Gray
  • Rick Rogers
  • Stephanie Grasson
  • (Glenn Research Center, Cleveland Ohio)
slide31

StereoImagingVelocimetry

The World's first 3D full-field quantitative and qualitative analysis tool that is portable and easily adaptable to shuttle experiments, ground-based investigations and industrial applications.

US Patent #5,905,568 - Stereo Imaging Velocimetry (1999)

US Patent #6,603,535 - Stereo Imaging Velocimetry system and method (2003)

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Stereo Imaging VelocimetryGoals and ObjectivesStudy low velocity fluid/air flow problemsProduce 3D quantitative data from experimentsSIV applications package - PC Based

Stereo Imaging Velocimetry is a system used to track the motion of particles in a transparent liquid in three dimensions. SIV consists of two cameras, oriented at 90o with respect to each other, observing a fluid experiment which has been seeded with small tracer particles. Each camera will record two dimensional data of the motion of the seed particles in the observation volume. Three dimensional data is obtained by computationally combining the two dimensional information.

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Stereo Imaging Velocimetry

The two cameras are set up perpendicular to each other, so that two widely disparate views are recorded. The two views are computationally combined to obtain three dimensional coordinates of the seed particles.

slide34

SIV Benefits and Potential Uses

  • Benefits:
  • Provides accurate, reproducible quantitative flow measurements
  • Three Dimensional
  • Uses No Lasers
  • Safe and Affordable
  • Utilizes "off-the-shelf" CCD cameras and PC workstation hardware
  • Unique tool for direct comparison of computed and experimentally measured fluid flows
  • No limitation on fluid flow scale to be measured (microscopic to macroscopic)
  • Velocities directly comparable to computational models
  • Potential Uses:
  • Combustion intake, compression, expansion, exhaust studies
  • Air flow studies around buildings
  • Improved aerodynamics of automobiles and aircraft
  • Avoiding "no flow" regions in artificial hearts
  • Analysis of crash dummy motion
  • Modeling of continuous casting operations (steel, nonferrous alloys)
  • Quieter airflow within auto heating & cooling ducts
  • More efficient HVAC
slide35

SIV Nomenclature

Right camera perspective (the left camera perspective is analogous)

xi yi zi = Absolute x y z coordinates of particle i.

XRi ZRi = Window coordinates of particle i on the face of the chamber.

xRi zRi = Pixel coordinates of particle i as seen by the right camera.

fR = Effective focal length of the right camera.

DR = Effective distance between the right camera and the face of the chamber.

dR = Horizontal distance of the right camera axis from the origin.

R = Vertical distance of the right camera axis from the origin.

CR = Camera dependent constant with the units mm/pixel.

slide36

SIV Consists of Five Main Phases:

1) Camera Calibration

2) Centroid Determination with Overlap Decomposition

3) Particle Tracking

4) Stereo Matching

5) 3D Analysis

slide37

SIV 3D Vectors

Top View

Side View

slide38

Structure Of Flame Balls At Low Lewis-number (SOFBALL)

SIV has been used for three Space Shuttle missions, providing analysis for the Combustion Module 1 (STS-83 and STS-94) and Combustion Module-2 experiments (STS-107 – Columbia) on a the SOFBALL (Structure Of Flame Balls At Low Lewis-number) project. SIV was used for over 50 Combustion Module experiments analyzing and identifying the 3D positions of flameball data.

slide39

MOBI experiment

SIV is currently being used to identify and track the motion of bubble droplet formations in a viscous fluid. The results will be used to improve the understanding of multiphase flows relevant to oil wells, bubble segregation in bioreactors and effect on oxygen transport to cultivated cells.

slide40

NCharge

The primary goal of this effort is to develop a technique that allows measurement of the electrostatic (or coulombic) charge on individual grains of material with diameters in the range of 50 to 500 microns by utilizing a microgravity environment.

slide41

Space Act Agreement with LTV Steel

Funded by TU Office

· Provide a diagnostic tool for quantitative and qualitative characterization of fluid flows.

· Permit direct comparison between computed and experimentally measured 3-D flows.

· Provide vector maps of the mold flow.

· Provide verification for mathematical models.

· PC-based SIV applications package, available for incorporation into fluid experiments.

· Help develop new nozzle designs to reduce defects.

Raw Data

Continuous Casting Model

SIV Vectors

Continuous Casting Model

slide42

Space Act Agreement with Kirby Vacuum Cleaner Company

  • Investigating the brush roll design for the next generation Kirby model.
  • Producing 3D velocity vectors of air flow studies.
  • Must use high speed video equipment.
slide43

A Compact Video Microscope Imaging System With Intelligent Controls (CMIS)

StereoImagingVelocimetryUS Patent #5,905,568 - Stereo imaging velocimetry (1999)

US Patent #6,603,535 - Stereo imaging velocimetry system and method (2003)

The World's first 3D full-field quantitative and qualitative analysis tool that is portable and easily adaptable to shuttle experiments, ground-based investigations and industrial applications.

byDr. Bilal Mark McDowell Bomani

NASA Glenn Research Center