Screening Methods. Lecture – 4 Tahir. Early screening strategies tends to be empirical, labour intensive and low success rates. As the no of commercially important compounds isolated increased, the success rates of such screens decreased further.
Lecture – 4
Early screening strategies tends to be empirical, labour intensive and low success rates.
Antibiotics initially detected by growing on ager plates to check the antimicrobial action.
Increasing frequency of penicillin and cephalosporin resistance amongst clinical bacteria led to the development of mechanism based screens for the isolation of more effective antibacterials.
The concept of using the inhibition of enzymes as a screening mechanism was pioneered by Umezawa 1972.
The detection of pharmacological agents by receptor ligand binding assay has been developed rapidly by pharmaceutical companies (Bull 1992). These are very effective even at low concentration