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Purposes:

Purposes: To demonstrate the tendency of proteins to become longer with increase of organism complexity To study domain architecture of proteins and to date different domains. Homologous proteins. Orthologs. Paralogs.

alphonse-meller
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Purposes:

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  1. Purposes: • To demonstrate the tendency of proteins to become longer with increase of organism complexity • To study domain architecture of proteins and to date different domains

  2. Homologous proteins Orthologs Paralogs Have evolved by vertical descent from a common ancestor and are presumed to have complete structural and functional correspondence Arise by duplication and domain shuffling within a genome and hence may have divergent functions New function We are interested in proteins that have changed their domain content, but preserved the same function

  3. Human HRX protein PHD zf-CXXC SET FYRN FYRC BROMO

  4. TUDOR PWWP HMG RRM zf-C4 zf-CXXC PHD BROMO SET PostSET FYRN FYRC Plant gi|15217143|gb|AAK92531.1|AF401284_1 trithorax 3 [Arabidopsis thaliana] (330 letters) gi|15233199|ref|NP_191733.1| putative protein [Arabidopsis thaliana] (902 letters) gi|6850313|gb|AAF29390.1|AC009999_10 Contains similarity to MLL proteinfrom Fugu rubripes gb|AF036382, and contains a PWWP PF|00855 and a SET PF|00856 domain. [Arabidopsis thaliana] (1193 letters) gi|15225109|ref|NP_180721.1| putative SET-domain transcriptional regulator [Arabidopsis thaliana] (186 letters) gi|15238735|ref|NP_200155.1| putative protein [Arabidopsis thaliana] (1040 letters) gi|16118405|gb|AAL12215.1| trithorax 4 [Arabidopsis thaliana] (285 letters) gi|15238953|ref|NP_199055.1| putative protein [Arabidopsis thaliana] (1421 letters) gi|15231914|ref|NP_187459.1| unknown protein [Arabidopsis thaliana] (764 letters)

  5. TUDOR PWWP HMG RRM zf-C4 zf-CXXC PHD BROMO SET PostSET FYRN FYRC Yeast gi|7493085|pir||T41282 probable transcription silencing protein - fission yeast (Schizosaccharomyces pombe) (920 letters) gi|6321911|ref|NP_011987.1| Gene has a 'SET' or 'TROMO' domain at its carboxyterminus like the trithorax gene family from human and Drosophila with postulated function in chromatin-mediated gene regulation.; Set1p [Saccharomyces cerevisiae] (1080 letters) Worm gi|17552318|ref|NP_498040.1| C26E6.9a.p [Caenorhabditis elegans] (1507 letters) + gi|17552316|ref|NP_498041.1| C26E6.9b.p [Caenorhabditis elegans] (739 letters) gi|17555046|ref|NP_499819.1| PHD-finger. (2 domains), SET domain [Caenorhabditis elegans] (2561 letters)

  6. TUDOR PWWP HMG RRM zf-C4 zf-CXXC PHD BROMO SET PostSET FYRN FYRC gi|7289568|gb|AAF45425.1| CG17396 gene product [Drosophila melanogaster] (177 letters) gi|7291672|gb|AAF47094.1| CG5591 gene product [Drosophila melanogaster] (630 letters) Fly gi|17861882|gb|AAL39418.1| GM10003p [Drosophila melanogaster] (421 letters) gi|7511805|pir||T12687 ALR protein homolog - fruit fly (Drosophila melanogaster) (2422 letters) gi|469801|emb|CAA83515.1| predicted trithorax protein [Drosophila melanogaster] (3358 letters) gi|15292119|gb|AAK93328.1| LD39445p [Drosophila melanogaster] (700 letters) gi|10720313|sp|Q24742|TRX_DROVI Trithorax protein (3828 letters)

  7. TUDOR PWWP HMG RRM zf-C4 zf-CXXC PHD BROMO SET PostSET FYRN FYRC gi|4588363|gb|AAD26112.1|AF105280_1 myeloid/lymphoid leukemia 2 [Homo sapiens] (140 letters) Human gi|1170364|sp|Q03164|HRX_HUMAN Zinc finger protein HRX (ALL-1) (Trithorax-like protein) (3969 letters) gi|4336749|gb|AAD17932.1| myeloid/lymphoid leukemia 2 [Homo sapiens] (1010 letters) gi|6634011|dbj|BAA20763.2| KIAA0304 protein [Homo sapiens] (1900 letters) gi|14424624|gb|AAH09337.1|AAH09337 Similar to KIAA0304 gene product [Homo sapiens] (798 letters) gi|13938427|gb|AAH07353.1|AAH07353 Similar to KIAA0304 gene product [Homo sapiens] (140 letters)

  8. TUDOR PWWP HMG RRM zf-C4 zf-CXXC PHD BROMO SET PostSET FYRN FYRC gi|16163206|ref|XP_037523.2| KIAA1076 protein [Homo sapiens] (772 letters) Human - continued gi|4505197|ref|NP_003473.1| mixed-lineage leukemia 2; ALL1-related gene [Homo sapiens] (5262 letters) gi|7512280|pir||T03455 ALR protein - human (4957 letters) gi|6683126|dbj|BAA20797.2| KIAA0339 protein [Homo sapiens] (1709 letters) gi|10864041|ref|NP_067053.1| mixed-lineage leukemia 3; ALR-like protein [Homo sapiens] (4025 letters) gi|10434227|dbj|BAB14179.1| unnamed protein product [Homo sapiens] (452 letters)

  9. TUDOR PWWP HMG RRM zf-C4 zf-CXXC PHD BROMO SET PostSET FYRN FYRC Vertebrates Homo sapiens 940Ma Arthropods Drosophyla melanogaster Drosophyla virilis 1087Ma Nematodes Caenorhabditis elegans Fungi Schizosaccharomyces pombe Saccharomyces cerevisiae 1508Ma Protista Plants Arabidopsis thaliana

  10. Conclusion: • Elongation of proteins within interspecific trx family is correlated with organism complexity • Proteins elongate following domain duplication, shuffling and accretion

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