Nucleus basics (pages to 6 th ed.). 1. Structure of DNA: 387-390, fig. 10.10. 2. Components of Chromosomes: origins (p. 547), centromeres(496), genes, telomeres (493):
1. Structure of DNA: 387-390, fig. 10.10
Circular bacterial replication- semiconservative, bidirectional
Three main types of RNA’s: mRNA, rRNA, tRNA, plus other small RNA types (421)
3 types of RNA polymerases & their products, use of alpha-amanitin to distinguish between the types. (443; my notes)
Promoters, transcription factors , enhancers, (435), Figs 11.6, 18
RNA processing: 5’ caps, Splicing, 3’ polyA tails, introns, and exons (437), Figs 11.21-23,
Ribosomal RNA- multiple copies of rRNA genes, rRNA formation from a single transcript by nucleolytic processing. (11.12, 14 p. 430)
Large and small ribosomal subunits; Start (met) and stop codons; translation beginning at 5’ end; translation beginning at the amino end, concluding at the COOH end. (455-467)
Cool experiment! ONE AA change, and it no longer goes to the nucleus!!!
It’s not really clear how RAN gets back in; it may simply diffuse in. Once in, it is reconverted to RAN-GTP. And, of course, the exportin needs to get back in as well!
A MAJOR export is mRNA! The splicing complex is involved in export! The Exon Joining Complex binds to TAP, which allows binding to the NPC and export. The EJC and TAP must then get back in.
Toxic legacy.In a controversial finding, exposing a pregnant rat to a toxin had health effects for three generations.
J Kaiser Science 2014;343:361-363
Published by AAAS
1. What is the complementary DNA sequence from this RNA sequence?
2. A polypeptide of _____ Amino acids could be coded for by this mRNA
3. Which end would be the carboxyl end of that polypeptide? (right or left end)
5. What’s this, and is the cytoplasm up or down?