1 / 26

Epithelio-mesenchymal interaction: kidney development

Epithelio-mesenchymal interaction: kidney development. Dr. Nandor Nagy. Semmelweis University, Budapest. Congenital polycystic disease of kidney (1:800 live birth), PKD1 and PKD2 mutation. Three sets of nephric systems:. Pronephros (Greek: first kidney). Urogenital ridge. 2. Mesonephros.

abrase
Download Presentation

Epithelio-mesenchymal interaction: kidney development

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Epithelio-mesenchymal interaction: kidney development Dr. Nandor Nagy Semmelweis University, Budapest

  2. Congenital polycystic disease of kidney (1:800 live birth), PKD1 and PKD2 mutation

  3. Three sets of nephric systems: • Pronephros (Greek: first kidney)

  4. Urogenital ridge

  5. 2. Mesonephros • Functionbetweeweek 6-10. • Mesonephricnephronsdonotformany part of thematurekidney !

  6. 2. Mesonephros

  7. 3. Metanephros (definitive kidney) Ureteric buds sprout out from the mesonephric ducts (E28) And penetrate into the metanephric blastema

  8. CHRONOLOGICAL ORDER OF THE DEVELOPMENT OF PRO-, MESO- AND METANEPHROS 1. 2. 3. 4. Cytokeratin fluorescence immunostaining of mouse embryo. Cytokeratin is present in nephric duct and its derivates. 20. day of embryonic life 25. day of embryonic life 35. day of embryonic life

  9. Ureteric bud Uretericbud – outgrowthfromthemesonephricductnearitsentranceintothecloaca • reciprocal induction -retinoic acid dependent • Cranial-caudal patterning establishes  a “renogenic” region within the intermediate mesoderm in the tail of the embryo –this renogenic mesoderm is the METANEPHRIC BLASTEMA • The METANEPHRIC BLASTEMA secretes growth factors that induce growth of the URETERIC BUD from the caudal portion of the mesonephric duct. • The URETERIC BUD proliferates and responds by secreting  growth factors that stimulates proliferation and then differentiation of the metanephricblastema into glomeruli and kidney tubules (i.e. induces the blastema to undergo mesenchymal-to-epithelial transition ).

  10. Ureteric bud Ureteric bud - elongates and penetrates the blastema 1. Caudal part -the stalk develops to ureter -reciprocalinductivesignals!!! 2. Cranial part - undergoes a number of dichotomousbranchingevents to form the urinary collecting tubulesystem -thefirst 4 generations of tubulesenlargetoformthemajor calyces - second 4 generationsfusetoformtheminor calyces - the end of eacharchedtubuleinducesclustersofblastemacellstoformsmallmetanephricvesicles

  11. GDNF expresszió

  12. -reciprocal inductive signals!!!

  13. Nephron formation 1.

  14. Cloacal plate

  15. Division of the cloacal region 5 weeks 6 weeks 8 weeks

More Related