rotifer ppt

1. VARIOUS METHODS FOR THE CULTURE OF THE ROTIFER Submitted by AbhishekGiri M.F.Sc. (4thSem) Dept. of Zoology and Applied Aquaculture Barkatullah University Bhopal, Madhya Pradesh 462026

2. CONTENTS • INTRODUCTION • METHODS • MAIN SPECIES OF ROTIFER USED FOR • REARING LARVAL FISH • 4. CULTURE METHODS • * METHODS USING BAKER’S YEAST AND FISH OIL • * METHOD USING ALGAE, YEAST AND FISH OIL • 5. FEEDING ROTIFERS TO LARVAL FISH • 6. CONCLUSION • 7. REFERENCES

3. INTRODUCTION • The rotifer, Brachionusplicatilis, is an important food organisms for the first feeding stages of larval marine animals around the world. • It is a cosmopolitoneuryhaline species, thus it is very versatile in marine culture. • The rotifer varies in size depending on culture conditions . • Adult size ranging from 123 to 315 µm in length. • It can be grown in seawater in a wide range of salinities. • Feed types vary, but the most common are algae such as Tetraselmis, Chlorellaand Isochrysis. • Other’s incluidbaker’s and torula yeast, fish oil and artificial diets.

4. METHODS • Stock cultures for inoculant or starter cultures are maintained in 1-2 liter flasks. • Stock cultures can be maintained on an algal diet of Isochrysisgalbana at 24-25℃and a light cycle of 12 light : 12 dark. • Cultures should be restarted periodically, at least every month or more.

5. MAIN SPECIES OF ROTIFERS • Brachionusplicatilis. (Marine Water) 2. Brachionusrotundiformis. (Marine Water) 3. Brachionuscalyciflorus. (Fresh Water)

8. CULTURE METHODS • Rotifers have been cultured in 1.8 m dia., round, flat-bottomed tanks that hold up to 1800 liters of water, 140 liter conical tanks; 160 liter clear, cylindrical fiberglass tanks; 50 liter polyethylene bags; and 1.8 m dia. 3200 liter round outdoor tanks. • Tanks are sterilized before use by addition of 2.5 ppm bleach for 12-24 hours. • They are then filled with filtered seawater and salinity is adjusted . • Temperature is maintained at 24-26℃. • Conical (room ceiling light) and outside tanks(sunlight) have continuous light supplied from light banks.

9. Using baker’s yeast and fish oil • Set up 1800 liter round tank or conical tanks. • On day one yeast is fed at 0.6-0.8 g/l, fish oil at 1.0 ml/10 l. • On day two yeast is fed at 1.5 g/106 rotifers and fish oil at 3ml/106 rotifers daily until rotifer density reaches 50/ml. • Then add 1-1.3 g yeast/106and 2-3 ml of fish oil/106 rotifers daily until a density of 100/ml is attained. • Then fed yeast at 0.6-1.0 g/106 rotifers and fish oil at 2ml/106 rotifers until the density reaches 150-200/ml. • At this time harvesting may begin.

11. Using algae, yeast and fish oil • Set up one 1800 liter tank and inoculate with a 12 liter carboy of Isochrysisgalbana(132,000 cells/ml) and medium (0.2 ml/liter of F/2 medium). • On the second and third days add 0.2 ml/lit of F/2 medium and when the algae density reaches 132,000 cells/ml inoculate with 1-10 rotifers/ml. • When the concentration of algae decreases, begin adding yeast at 50 g/tank and fish oil at 1-2 ml/10 liters each day. • When rotifer density reaches 100/ml or more, begin adding yeast at 0.7-1.0 g/106 rotifers and fish oil at 2-3 ml/106 rotifers. • Harvesting rotifers can begin when the density reaches 200 rotifers/ml.

13. FEEDING ROTIFERS TO LARVAL FISH • Larval red drum begin feeding approximately three days after hatching, when their mouth parts develop. • Rotifers are fed at this time at a rate of 3-5 rotifers/ml until larger fed can be consumed. • Due to the loss in nutritional value a few hours after harvest, it is best to feed rotifers to fish at list twice a day.

14. CONCLUSION • There are many ways to culture rotifers. • Keep culture containers and water clean. • Control contaminants such as ciliates and bacteria. • Harvest daily to maintain the culture in growth phase. • Add some algae daily, even a small amount.

15. REFERENCES • A text book of fish and fishery science – C.B.L.Srivastava. • An introduction to fishes – S.S.Khanna. • Work book on Limnology – A.D.Adoni.

16. Thank you