Magnification of microscope

1. Microscopes!

2. Mutual dependence: Science & Technology • Technology…the pragmatist • Does it work? • Is it useful? • Is it sustainable? • Science…the idealist • What is it? • How does it work? • What does it tell us • Science: evidence, rationality and the quest for deeper understanding • Tech: practical, appropriare and useful and an increasing emphasis on sustainability

3. Mutual dependence: Science & Technology • Microscopes • Electron microscopes • Optical (light) microscopes

4. Size matters • micron = micrometer = 1/1,000,000 meter • diameter of human hair = ~20 microns Miss N, Show them the interactive!!

5. Microscopic units of measurement

6. 1 metre (m) = ________ millimetres (mm) 1 mm = ________ microns (µm) 1 µm = ________ nanometres (nm) 1 mm = ________ nm 1 m = ________ µm 1 m =________ nm

7. 1 m = 1,000 mm = 103mm 1 mm = 1,000 µm= 103µm 1 µm = 1,000 nm= 103nm 1 mm = 1,000,000 nm = 106nm 1 m = 1,000,000 µm = 106µm 1 m = 1,000,000,000nm =109 nm

8. 10-3 m = ________ 10-6 m = ________ 10-9 m = ________

9. 10-3 m = 0.001m = 1 mm 10-6 m = 0.000001 = 1 µm 10-9 m = 0.000000001= 1nm

10. Unaided? Electron microscope range: _____ to _____ Light microscope range: _____ to _____ Unaided human eye range: _____ to _____

11. Electron microscope range: 1 nm to 100 µm Light microscope range: 1 µm to 1 mm Unaided human eye range: 1 mm upwards

12. Name that image Light microscope or Electron microscope?

13. 19

14. Unstained Light microscope 19

15. 20

16. Light microscope 20

17. 21

18. 22

19. Light microscope 22

20. Tick 23

21. Tick Electron microscope 23

22. 24

23. Electron microscope 24

24. 25

25. Light microscope 25

26. 26

27. Electron microscope 26

28. Aphid 27

29. Aphid Electron microscope 27

30. 28

31. Light microscope 28

32. 29

33. Light microscope 29

34. 30

35. Electron microscope 30

36. 31

37. Electron microscope 31

38. 32

39. Electron microscope 32

40. 33

41. Electron microscope 33

42. 35

43. Light microscope 35

44. 36

45. Light microscope 36

46. 37

47. Light microscope 37

48. Magnification Magnification = ocular lens x objective lens A microscope has an eyepiece lens with a power of 20X.  The objective lens being used has a power of 10X. What is the total magnification?

49. Using Scale Bars Using a scale bar: The image at right is of a virus-like particle. The bar represents 50nm. Calculate the actual size of the image. 50nm

50. Using Scale Bars Using a scale bar: • Measure the scale bar • 50nm=1cm • 2. Measure the image • Let’s say it was 2cm. • Actual size: Image size = constant • 50:1 = X:2 • X=2*50 • =100nm 50nm