1. Fig. 11-CO, p.264

2. Learning Objectives • How Does Transcription Take Place in Prokaryotes? • How Is Transcription Regulated in Prokaryotes? • How Does Transcription Take Place in Eukaryotes? • How Is Transcription Regulated in Eukaryotes? • How Is RNA Modified after Transcription? • How Does RNA Act as an Enzyme?

5. Table 11-1, p.265

6. The basics of transcription Fig. 11-1, p.265

7. Sequence of representative promoters from E.coli Fig. 11-2, p.266

8. Sequence of events in the initiation & elongation phases of transcription in prokaryotes Fig. 11-3, p.268

9. Inverted repeats terminate transcription Fig. 11-5, p.269

11. The rho(p)factor mechanism of transcription termination Fig. 11-6, p.270

12. Control of transcription via different σ subunit c Fig. 11-7, p.271

13. Elements of a bacterial promoter Fig. 11-8, p.271

14. The mode of acion of the lac repressor Fig. 11-9, p.273

15. Basic control mechanisms seen in the control of genes Fig. 11-12, p.275

16. The attenuation mechanism in the trp operon Fig. 11-15, p.277

18. Summary of eukaryotic transcription • It is more complicated than prokaryotic transcription • 3 RNA polymerases of which Pol II produces mRNA • The organization of promoters & enhancers is more complicated • An impoatant element is TATA box at -25 • 6 general initiation factors are involved in forming the initiation complex

19. F Four elements of polymerase II promoters in eukaryotes Fig. 11-17, p.280

21. DNA looping brings enhancers in contact with transcription factors and RNA polymerase Fig. 11-20, p.285

22. Table 11-4, p.286

23. Activation of transcription via CREB & CBP Fig. 11-21, p.286

24. Multiple ways in which CREB binding protein (CBP) and p300 are involved in gene expression Fig. 11-22, p.287

25. Table 11-5, p.287

29. Posttranscriptional modification of a tRNA precursor Fig. 11-30, p.292

30. The organization of split genes in eukaryotes Fig. 11-33, p.294

31. Splicing of mRNA precursors ( a lariat forms in the intron ) Fig. 11-34, p.295

32. Organization of the fast skeletal muscle troponin T gene and the 64 possible mRNAs that can be generated from it The exons in blue and red are exclusive .only one or the other may be used Fig. 11-35, p.296

33. SLE : Systemic lupus erythematosus is an auto immune disease Production of a.b. to one of the snRNPs,U1-snRNP Rash on the forehead & cheek bones, giving the wolf like appearance. Severe kidney damage may follow with arthritis, accumulation of fluid around the heart, & inflammation of the lungs. L SLE p.296

34. END Chapter 11