Annual Refresher Centrifugation Hazards. Centrifugation 101. Every time you use a centrifuge, you make series of choices. Which centrifuge, which rotor, which tubes and adapters, what speed, for how long, what level of containment.
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Every time you use a centrifuge, you make series of choices. Which centrifuge, which rotor, which tubes and adapters, what speed, for how long, what level of containment.
If you are unsure of your choice, ask an experienced colleague, consult the operating manual, or call the manufacturer’s representative.
Before placing the rotor in the centrifuge, make sure the bowl is dry and the drive spindle is clean
Balance tubes and bottles carefully and avoid overfilling. If tubes used in fixed angle rotors, centrifugal force drives the fluid up the outside tube wall.
Make sure the rotor is seated on the drive hub correctly
Don’t overload beyond the rotor’s maximum mass without reducing the rated speed
When using a horizontal rotor, make sure all buckets are hooked correctly and move freely
Check O-rings on containers and rotors for cracks, nicks, or chemical attack. Apply vacuum grease to the seals at least weekly.
Common user errors are failure to secure the rotor to the drive, failure to put the lid on the rotor, and failure to secure the lid.
It is good practice to stay at the centrifuge until it is running smoothly at the desired run speed.
When the run is complete, do not open door until rotor has come to a halt. Always check for possible spill. If you find one, be sure to clean both the centrifuge and rotor thoroughly
Integrity of rotors can be compromised by corrosion or fatigue.
Make sure you fill out the quiz, sign it, and drop it off in my mailbox for record keeping.
Remember: This is an annual requirement.
SEE YOU NEXT YEAR
Contact: Kathie Moh