The Cell:. DNA. gene. Nucleus. mRNA. Transport. Cytoplasm. Protein. ori. bla. What is a plasmid?. Circular piece of autonomously replicating DNA. Originally evolved by bacteria May express antibiotic resistance or be modified to express proteins of interest. araC. ori. pGLO.
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1. Incubation on ice slows fluid cell membranes
2. Heat-shock increases permeability of cell membrane
3. LB recovery allows beta lactamase expression