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Bioseparations engineering

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Bioseparations engineering

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    1. Bioseparations engineering Definition: Recovery, isolation, purification and polishing of products synthesized by biotechnological processes Extended definition: Final polishing steps of processes such as biotechnology based effluent treatment and water purification

    3. Challenges in bioseparations engineering

    4. Biological products

    5. Biological products (contd..)

    6. A good bioseparation process: Ensures desired purity of product Ensures stability of product Keeps cost low Is reproducible Is scalable Meets regulatory guidelines

    7. Economic importance of bioseparation engineering

    8. Strategies for bioseparation A large number of bioseparation methods are available The strategy is based on how best these can be utilized for a given separation The following need to be taken into account: The volume of process stream The relative abundance of the product in this process stream The intended use of the product, i.e. purity requirements The cost of the product Stability requirements

    9. Conventional strategy: The RIPP scheme Recovery, isolation, purification and polishing Based on a logical arrangement of bioseparation methods Low-resolution, high-throughput techniques (e.g. precipitation, filtration, centrifugation, crystallization) are first used for recovery and isolation High-resolution techniques (e.g. adsorption, chromatography, electrophoresis) are then used for purification and polishing It is now possible to avoid this RIPP scheme

    10. Bioseparation methods Low resolution-high throughput Cell disruption Precipitation Centrifugation Liquid-liquid extraction Leaching Filtration Supercritical fluid extraction Microfiltration Dialysis High resolution-low throughput Ultracentrifugation Adsorption Packed bed chromatography Affinity separation Electrophoresis

    11. Bioseparation methods (contd..) High resolution-high throughput Ultrafiltration Fluidized bed chromatography Membrane chromatography Monolith column chromatography In this course we will discuss: Ultrafiltration Membrane chromatography (membrane adsorption)

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