Enzymes with recognition sequences from 4 to 8 nucleotides in length each have uses in genetic engineering. 6-cutters (i.e. enzymes that have ...
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EcoRI restriction enzyme
Frequencies of recognition sites:
4 bp: 44 = 256 nt
6 bp: 46 = 4096 nt
8 bp: 48 = 65536 nt (NotI cuts E. coli chromosome 21 times)
Pingoud & Jeltsch (2001) Nucl. Acid Res. 29: 3705-3727.
Digest of original plasmid
-agarose or polyacrylamide