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Target X-ray Crystallography

Target X-ray Crystallography for students,scholars,researchers...

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Target X-ray Crystallography

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  1. Target X-ray Crystallography

  2. Obtaining the accurate three-dimensional (3D) structure information of the target is helpful for the discovery and optimization of drug candidates, as well as the mechanism of action (MOA) studies. As a leading service provider in the field of structural biology, relying on the first-class structural biology technology platform that covers X-ray crystallography, cryo-electron microscopy (Cryo-EM) technology and protein NMR spectroscopy, Creative Biostructure provides a comprehensive solution of target structure determination, called MagHelix™ Gene-to-Protein and Gene-to-Structure, complemented with the most advanced biophysical methods to characterize target and target-ligand interaction. Our services support structure-guided lead discovery and optimization, as well as biological drug discovery, such as therapeutic antibody discovery.

  3. High Quality Protein Production • 1. Molecular cloning

  4. Our molecular cloning service includes the design and preparation of expression constructs. We provide customers with all commercially available vectors for construct design. Various purification tags are supplied for constructing recombinant plasmids, such as SUMO, MBP, GST, His tags, etc. We also have various inserts such as GPCR, kinases, phosphatases, and epigenetic targets. In this process, our scientists will determine the appropriate construct boundaries according to experience, precedent, and specific research applications and propose the optimal expression strategy. Our experienced technicians are able to perform site mutation, deletion, and multi-parallel construct design equipped with advanced techniques.

  5. 2. Target protein expression and purification • The service includes recombinant protein expression testing, optimization of protein expression and purification, as well as large-scale protein overexpression and chromatographic purification. Creative Biostructure provides customers with high-performance protein expression systems including E. coli, yeast, insect cells, and mammalian cells. We purify the target protein using the AKTA FPLC systems with multiple chromatographic methods (our standard protocol includes affinity chromatography, ion-exchange chromatography, and size-exclusion chromatography). After purification, the protein can be tested with UV spectrophotometry, SDS-PAGE, and western blot to ensure > 95% purity. The service is available as a stand-alone one or in combination with downstream applications. It is worth mentioning that we support custom membrane protein production.

  6. Related Services: • Structural Modeling of Target Molecule

  7. Excerpt from: https://drug-discovery.creative-biostructure.com/maghelix-gene-to-protein-and-gene-to-structure-p15

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