VIRAL DNA PURIFICATION “Trino’s Lab” Page 209-231. FAMILY: Geminiviridae Genus: Begomovirus Species: Tomato golden mosaic virus (TGMV ). Properties of DNA. Large polymers made up of nitrogenous bases and 5 carbon sugars linked by phosphodiester bonds.
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Species: Tomato golden mosaic virus (TGMV)
Average Amax is at A260
Viral DNAGEMINIVIRUS DNA PURIFICATION
End of Day One
Membrane fixation, Probe preparation, Hybridization
M I I U U M
M=marker, I= infected, U= uninfected, Arrow indicates virus RNA
This is a Southern hybridization
DNA to DNA. Probe binds by base pairing
with Denatured DNA on the nylon filter
An antibody can detect the DU labled probe
Identification of Virus DNA in mixture of host cell DNA by antibody binding to DU labled Gemini specific probe
I = Infected
M I M I