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Subduction Behaviors Generates Tension in Lateral Marginal Zone (LMZ) Explants PowerPoint Presentation
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Early Gastrula Subduction Behaviors Generates Tension in Lateral Marginal Zone (LMZ) Explants Apical constriction can pull: A. mexicanum LMZ “Tractor Pull” Animal edge of free coverslip fragment edge of attached coverslip fragment Onset of apical constriction

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Presentation Transcript
slide1

Early Gastrula

Subduction Behaviors Generates Tension in Lateral Marginal Zone (LMZ) Explants

slide2

Apical constriction can pull:

A. mexicanum LMZ “Tractor Pull”

Animal

edge of free coverslip fragment

edge of

attached

coverslip

fragment

Onset of apical constriction

= initial lateral blastopore

Vegetal

100 minutes/second; 7 hours elapsed time

slide3

Handle

Explant

Sled

XYZ pos

CS

Probe

… And I Can Measure that Force

Schematic for LMZ explant in Tractor Pull assay

View from Apical side of Ambystoma AC explant in Tractor Pull assay