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Functional and Structural Repair of Peripheral Nerve Injury by Adipose-Derived Stem Cells: An Experimental Study. Hiroshi Mizuno, MD, A. Cagri Uysal, MD, Hakan Orbay, MD, Kyoko Kobe, MD, Hiko Hyakusoku, MD Department of Plastic and Reconstructive Surgery, Nippon Medical School
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Hiroshi Mizuno, MD,
A. Cagri Uysal, MD,
Hakan Orbay, MD,
Kyoko Kobe, MD,
Hiko Hyakusoku, MD
Department of Plastic and Reconstructive Surgery,
Nippon Medical School
Neural tissue must heal with true regeneration, because healing by scar will not re-establish electrical connectivity.
Adipose derived stem cells(ASCs) were proven to have a positive effect on skin wound healing processes.
Nambu M et al, Ann Plast Surg 62; 317, 2009
Clinical Treatment of Radiotherapy Tissue Damage by Adipose-Derived Adult Stem Cells
Rigotti G et al, Plast Reconstr Surg, 119: 1409, 2007
We have performed an experimental study on rats to find out the effect of adipose derived stem cells (ASCs) on primary peripheral nerve repair.
The ASCs were gathered from
After three passage in control medium
(DMEM, 10% FBS), the cells were
ready to be injected.
Every injection included 1x107 cells.
The cells were labelled with DiI and Hoechst staining before the injection for tracing. Every injection included 1x107 stem cells
Primary incision and nerve coaptation to sciatic nerve
Over the coapted nerve
Experimental: fibrin glue with ASCs
Control: fibrin glue only
MATERIALS AND METHODS
toe spread (ITS)
Toe spread (TS)
Print length (PL)
SFI=118.9 x TSF - 51.2 x PLF - 7.5
TSF (toe spread factor) = (TS experimental – TS normal) / TS normal
PLF (print lenghth factor ) = (PL experimental – PL normal) /PL normal
Inserra et al,
Sciatic function index (SFI) at 3rd month
Sciatic function index (SFI) at 6th month
Anti – S100& hematoxylen
RESULTS Histology Double positive endothelial(e) cells in the epineurium(EP)
DiI & hematoxylen x100
Hoescht & hematoxylen x100
A myelinated fiber indicates the presence of a schwann cell.
The myelinated vs total fiber percentage.(* p<0.05)