Hold micropipet and microtubes at eye level - PowerPoint PPT Presentation

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Hold micropipet and microtubes at eye level PowerPoint Presentation
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Hold micropipet and microtubes at eye level

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  1. Hold micropipet and microtubes at eye level

  2. Micropipet Use • Twist dial to desired volume • Add disposable pipet tip • Press plunger to first stop • Insert pipet tip into solution to be • transferred • Slowly release plunger to retrieve liquid • Move pipet tip into desired tube • 7 Gently place tip against side of microtube so adhesion forces can help in the ejection of desired volume. • 8 Press plunger past first stop to second stop • to transfer liquid, keep the plunger down • as you remove it from the tube. • 9 Eject tip

  3. Micropipetting technique A

  4. technique B Close the tips!

  5. Technique C Billiard style

  6. Micropipet tip should be ABOVE the well NOT IN IT!!!!

  7. Micropipet tip punched right through the gel See dye under the wells

  8. NICE!