Genetic Engineering. Biology Mrs. Appel Pgs 247-259. Breeding Strategies. Farmers and ranchers throughout history have tried to improve plants and animals by selecting the most desired organisms to breed. Selective Breeding : Mating individuals with a desired trait. Ex: milk cows
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Farmers and ranchers throughout history have tried to improve plants and animals by selecting the most desired organisms to breed.
-the process of isolating a desired gene from the DNA of one organism and transferring it to the DNA of another organism.
-Medicines, vaccines, gene therapy, agriculture,
-Genetic engineering experiments use different approaches , but most share 4 or 5 basic steps……….
(Gene of interest)
(Gene of interest)
back into bacterial cells by:
Makes it possible to produce large numbers of identical organisms with favorable genetic characteristics.
Hundreds of cloned animals exist today, but the number of different species is limited. Attempts at cloning certain species such as monkeys, chickens, horses, and dogs, have been unsuccessful.
Transgenic Plants: ex: insecticides, fertilizer, resistance to harsh environmental conditions and viruses, glowing plants??
Transgenic Animals: ex: growth hormone gene inserted in cattle and fish, farm animals resistant to certain diseases.
Would it be
Human Genome Project
A map of a portion of a human chromsome
Video on Genetic Engineering Applications
•1985- Alec Jeffreys introduced DNA fingerprinting. It is based on the fact that no two people (except identical twins), have the same DNA fingerprint.
•Uses: criminal investigations, paternity, rape cases, etc.
•Very small amounts of DNA are needed, taken from blood, saliva,hair, urine, etc.
Making A DNA fingerprint
DNA is cut by restriction enzymes at specific recognition sites.
DNA is micro-pipetted into the wells.
An electric current moves the DNA across the gel.
-DNA has a neg. charge and will travel towards the pos. end of the gel
-small fragments of DNA will travel further.
4. The DNA bands are compared.