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Tetrazine Reagents PowerPoint Presentation
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Tetrazine Reagents

Tetrazine Reagents

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Tetrazine Reagents

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  1. CLICKCHEMISTRYTOOLS.COM

  2. Click Chemistry Reagents Find and Shop Cu-Free Click, DBCO, TCO, tetrazine, azide, alkyne, and related Click Chemistry reagents. Order Now! Expand the boundary of your discovery research with our click chemistry toolbox. Find at clickchemistrytools.com for Click Chemistry Reagents.

  3. DBCO-PEG4-NHS Ester • DBCO-PEG4-NHS Ester reacts specifically and efficiently with a primary amine at pH 7-9 to form a covalent bond. Expand the boundary of your discovery research with our click chemistry toolbox. • View out wide selection of DBCO TAMRA with us. TAMRA DBCO dye reacts with azides via a copper-free "click chemistry" reaction to form a stable triazole! Expand the boundary of your discovery research with our click chemistry toolbox. • DBCO-PEG4-Bitoin reacts with azides via a copper-free "click chemistry" reaction to form a stable triazole. CLICKCHEMISTRYTOOLS.COM

  4. Properties of DBCO NHS ester • The properties of DBCO NHS ester are plenty. There offer: • Excellent biocompatibility • Strain promoted click reactions occur at a rapid pace when mild buffer conditions are created. There is no need to make use of catalyst during such reactions. • High chemo selectivity • DBCO ester doesn’t interfere or react with the functional groups that are naturally present in the biological samples. These, however, conjugate with a high efficiency with one another. • Amine Reactivity • DBCO TAMRA NHS ester can modify the molecules that contain amine. These are used for labeling with the counterparts of azide. CLICKCHEMISTRYTOOLS.COM

  5. The DBCO NHS ester is a simple reagent that posses a terminal carboxylic acid. This is activated as NHS ester. This can be used to react with free amine groups and helps in the formation of stable amid bonds. Because of the short space, the modified biomolecules experience an increase in the mass minimally. The DBCO TAMRA is not suitable for staining the intracellular components. The cells that have a higher degree of permeability are not advisable to be stained with this dye because it can make higher backgrounds that are not useful for the chemists. The intracellular components are kept safe from staining.

  6. Applications of DBCO NHS ester • 1. Protein-protein conjugation • 2. Dye labeling • 3. Protein immobilization and surface modification • 4. Radio labeling • 5. Protein-peptide conjugation • 6. Protein oligonucleotide conjugation • DBCO NHS esters are sensitive to moisture. In order to prevent the condensation of moisture onto any product it is vital to ensure that the vial comes down to the room temperature. You need to be careful while limiting exposure to the moisture and also while restoration under an inert atmosphere. The moiety hydrolyzes readily and tends to become non-reactive. Hence, it is vital to prepare the stock solutions just before use. When the stock solutions are prepared in solvents that are anhydrous, then this solution can be utilized for several days. It needs to be refrigerated when not in use. The reactions involving hydrolysis of the DBCO NHS ester is a competing reacting. Conjugation occurs with the primary amines of peptides or proteins.

  7. CLICKCHEMISTRYTOOLS.COM

  8. CLICKCHEMISTRYTOOLS.COM