1 / 2

malondialdehyde in plants

Lifeasible has developed a powerful plant transformation platform to generate genetically modified plants (also known as transgenic plants). We provide both transient and genetically stable transformation services in a wide range of plant species, including model plants, major food crops, economic plants, and new-era bio-energy feedstock plants (please see our plant species list for details).

Booten119
Download Presentation

malondialdehyde in plants

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Malondialdehyde in plants https://www.lifeasible.com/custom-solutions/pla nt/analytical-services/plant-physiology-analysis/ plant-stress-response-indicators-analysis/malon dialdehyde-content-measurement/ Malondialdehyde (MDA) is an organic compound with the nominal formula of CH2 (CHO)2. MDA can be generated by oxidizing agents that alter lipid structure, and cause lipid peroxidation of polyunsaturated fatty acids. MDA is one of the most widely measured non-enzymatically formed reactive electrophilic species (RES), and is usually used as a marker for oxidative stress. Lifeasible, as a leading plant biotechnology company, provides advanced technologies for MDA content measurement. The most commonly used methods for the determination of MDA content as follows (Figure 1): Figure 1. Simplified schematic descriptions of three different reactions that are utilized in MDA analysis (Tsikas, 2017). Thiobarbituric acid (TBA) reactive substances assay. This assay is based on the principle that under acidic conditions (e.g., glacial acetic acid or sulfuric acid) and elevated temperatures (e.g., 95 °C) for extended reaction time (e.g., 60 min), the MDA with TBA can form a red-colored MDA-(TBA)2 adduct (Figure 1A). The MDA-(TBA)2 adduct can be easily quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). Dinitrophenyl hydrazine (DNPH) assay. This assay utilizes derivatization reagents such as 2, 4- dinitrophenyl hydrazine (DNPH) or 2, 4-diaminonaphthalene (DAN) to react with the carbonyl groups of MDA (Figure 1B). The resulted complex can be quantified by HPLC, GC-MS, or LC-MS/MS methods. Pentafluorobenzyl (PFB) bromide assay. The PFB bromide can alkylate MDA at its central C atom, which causes both carbonylic groups to intact (Figure 1C). The MDA-(PFB)2 derivative can be quantified by GC-MS or GC-MS/MS analysis. We deliver consistent, high-quality data with the flexibility to adapt protocols based on specific customer requirements. Welcome to contact us for technical consults, quotations, questions, and more.

  2. Reference Tsikas D. Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples:Analytical and biological challenges. Anal Biochem. 2017, 524: 13-30.

More Related