Dependence of Corneal Stem Cells on Normal Ocular Surface Innervation. H. Ueno, G. Ferrari, T. Hattori, T. Funaki, D.R. Saban , S.K. Chauhan , R. Dana. Schepens Eye Research Institute, Harvard Medical School. The authors have no financial interest in the subject matter of this poster.
Dependence of Corneal Stem Cellson Normal Ocular Surface Innervation
H. Ueno, G. Ferrari, T. Hattori,
T. Funaki, D.R. Saban, S.K. Chauhan,
Schepens Eye Research Institute, Harvard Medical School
The authors have no financial interest in the subject
matter of this poster.
Neurotrophickeratopathy (NK) is a degenerative disease of the cornea characterized by impaired healing.
Corneal sensory nerve degeneration is responsible for corneal epithelial defects, stromal thinning, and perforation.
Seitz B, Kruse FE.
Trigeminal Stereotactic Electrolysis (TSE)
no blink reflex
１. Real Time-PCR
ABCG2, p63, Hes1
3. Flow cytometry
G. Ferrari et.al.
Expression of beta-III tubulin (neuronal marker) is decreased in the denervated cornea.
Expression of stem cell markers (ABCG2 & p63) is decreased in the denervated cornea.
RNA expression levels for both stem cell markers ABCG2 and Hes1 are significantly decreased in denervated corneas.
Relative Expression of mRNA
Corneal stem cells are significantly reduced in
this murine model of neurotrophickeratopathy.
Expression of stem cell markers (ABCG2, P63, Hes1) in
denervated corneas is decreased at both the mRNA and
Our novel data clearly demonstrate that
corneal nerves play a critical role in maintaining
the corneal stem cells.
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