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Kevin A. Negrete - President. DNA Workshop (Bologna, Italy). To live better …. longer. To increase the quality of life after 40. Two main factors contributing longer (historical) lifespan. Two main factors contributing to limited lifespan. reduce cellular oxidation .

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Kevin A. Negrete - President


DNA Workshop(Bologna, Italy)


To live better

To live better…

longer

To increase the quality of life after 40




reduce cellular oxidation

the formation of nascent oxygen (O2)

and the elimination of free radicals (O+)



Research has allowed scientists to identify central aging system in cells – and specifically the aging clock in DNA

The two basic DNA markers that regulate aging are

Telomere length and Methyl Group Loss


Telomere system in cells – and specifically the aging clock in DNA

Inside the center or nucleus of a cell, our genes are located on twisted, double-stranded molecules of DNA called chromosomes.

At the ends of the chromosomes are stretches of DNA called telomeres, which protect our genetic data, make it possible for cells to divide.

Telomeres have been compared with the plastic tips on shoelaces because they prevent chromosome ends from fraying and sticking to each other, which would scramble an organism's genetic information to cause cancer, other degenerative diseases, or death.

Each time a cell divides the telomeres get shorter (by 30-200 BP). Healthy cells only divide between 50-70 times. When telomeres get too short, the cells no longer can divide and becomes inactive, sustain genetic damage and ultimately die.


Similar to telomere shortening – the loss of Methyl Groups (CH3) shows a specific preprogrammed degradation throughout mammalian lifespan. The degradation is accelerated by oxidative stress, poor nutrition, lack of exercise, and environmental toxins.

H

H

H

H

H

H

H

H

H


Final Common Pathway of Aging – DNA Demethylation (CH3) shows a specific preprogrammed degradation throughout mammalian lifespan. The degradation is accelerated by oxidative stress, poor nutrition, lack of exercise, and environmental toxins.

10% loss

25 yrs

20% loss

50 yrs

30% loss

75 yrs

40% loss

Death

Birth


Understand true anti-aging (DNA) mammalian chemistry consists of 2 goals: elongate telomers and increase DNA methylation.

ENHANCED

NUCLEOTIDE

SUPPLEMENATION


Longevity of Control vs. Treated Animals consists of 2 goals: elongate telomers and increase DNA methylation.

2500

2000

1500

1500

1000

750

150

750

750

Control

Treated


Record mammalian longevity achieved through weekly injection of dna and rna
Record mammalian longevity achieved through weekly injection of DNA and RNA

  • Study began with 750 day old rats with usual longevity of 800 to 900 days

  • Identical conditions except treated animals received nucleic acid injections

  • Post 8 weeks control rats lost fur and vitality, whereas treated animals increased muscle mass, libido, and activity levels

  • Control animals all died in less than 150 days from start of study

  • Treated animals lived a minimum of 850 up to 1500 additional days from start of study, doubling and even tripling the usual life span


Understand true anti-aging (DNA) mammalian chemistry consists of 2 goals: elongate telomers and increase DNA methylation.

HOMOCYSTEINE


What is Homocysteine? consists of 2 goals: elongate telomers and increase DNA methylation.

Homocysteine is a toxic amino acid by-product produced by our own organism and accumulates in the bloodstream.

Elevated homocysteine is not only the indicator of, but is the CAUSE of many major diseases includinAlzheimer’, Arthritis, Cancer, Chronic Fatigue, Depression, Diabetes, Heart Attacks, Infertility, Obesity, Strokes, Thyroid Problems, and Ulcers

Homocysteine also accelerates the oxidation of LDL


Homocysteine Level VS Cardiac Risk Ratio consists of 2 goals: elongate telomers and increase DNA methylation.


Research has shown that higher HOMOCYSTEINE levels are directly

related to demethylation and telomere shortening

3 ways to detoxify HOMOCYSTEINE


Methyl group transfer metabolic pathways
Methyl Group Transfer directly Metabolic Pathways

Methionine

ATP

S-adenosyl-methionine (SAMe)

DMG

B12 Folic Acid

Betaine (TMG)

Donates CH3 to:

DNA

Proteins

Lipids

Carbohydrates

Myelin

Detox Pathways

Neurotransmitter Production

And others

S-adenosyl-homocysteine (SAH)

Choline

Homocysteine (Hcy)

Zn Vitamin B6

Cysteine

Glutathione


We have developed THE ENGINE OF REMETHYLATION directly

making cells younger through

Enhanced Nucleotide Supplementation


Methyl group transfer metabolic pathways1
Methyl Group Transfer directly Metabolic Pathways

Methionine

ATP

S-adenosyl-methionine (SAMe)

DMG

B12 Folic Acid

Betaine (TMG)

Donates CH3 to:

DNA

Proteins

Lipids

Carbohydrates

Myelin

Detox Pathways

Neurotransmitter Production

And others

S-adenosyl-homocysteine (SAH)

Choline

Homocysteine (Hcy)

Zn Vitamin B6

Cysteine

Glutathione


Could life-extension and anti-aging be as simple as directly

supplying the body with DNA and RNA?


Primary source of nucleotides is endogenous synthesis from amino acids and simple precursors
Primary source of nucleotides is endogenous synthesis from amino acids and simple precursors

  • Therefore nucleic acids have generally been considered nonessential nutrients

  • Under stress, however, some tissues unable to produce enough nucleotides to support tissue needs

  • In these conditions, nucleic acids bases may become essential nutrients for optimum tissue repair and function

  • Glycine, Serine, Aspartic Acid, Glutamine


Nucleic Acids : Food & Supplementation amino acids and simple precursors


So why doesn t everyone just take a giant nucleic acid pill
So why doesn’t everyone just take a amino acids and simple precursorsgiant nucleic acid pill?


Orally ingested nucleic acids undergo intensive metabolic degradation in upper gi tract
Orally ingested nucleic acids undergo intensive metabolic degradation in upper GI tract

  • Over 99% of purine bases oxidized to uric acid before entering portal circulation

  • About 95% of pyrimidines are metabolized before leaving intestinal lining

  • Only 3% of ingested pyrimidines reach liver


Photo Acoustic Resonance Laser degradation in upper GI tract


Laser Enhancement Technology degradation in upper GI tract

Nutrient molecules get distorted

during food or supplementation processing. Result: reduced nutrient assimilation by the cells.

Laser Enhancement Technology reshapes nutrient molecules.

Result: increased nutrient assimilation by the cells.


Laser Homogenized degradation in upper GI tractBetaine HCl Crystal

Control Betaine HCl


Extensive experimental and clinical evidence for benefit from nucleotide supplementation
Extensive experimental and clinical evidence for benefit from nucleotide supplementation

  • Increased survival in mice with Staph aureus bacteremia from 21% to 71%

  • Significantly increased survival in mice with Candida sepsis – greater effect with parenteral nucleotides than oral administration

  • Survival in animals with high dose radiation exposure increased from 5% to 50%


  • Single injection of RNA in mice post tumor immunization improved outcome from 0% to 40% long term survival

  • Rate of liver regeneration in rats post 70% hepatectomy significantly greater if IV nucleic acid bases given

  • In young rats with chronic diarrhea, appearance and enzyme function of intestine greatly improved

  • Improved growth and maturation in young animals


  • Infants given formula supplemented with nucleotides to mimic content of breast milk showed more benevolent intestinal flora an less diarrhea (50 times more nucleic acids in breast milk vs. formula which is significant in developing countries)

  • Supplemented infants had better lipid profiles with higher HDL cholesterol

  • Significant improvement in human cellular immune function

  • Significant improvement in memory in animals and humans

  • Accelerated wound healing


Atp delivery specifically associated with numerous benefits
ATP delivery specifically associated content of breast milk showed more benevolent intestinal flora an less diarrhea (50 times more nucleic acids in breast milk vs. formula which is significant in developing countries)with numerous benefits

  • Improved pulmonary function, even in cystic fibrosis

  • Enhanced cardiac function – strengthening the heart

  • Relief of nerve pain and improved nerve conduction

  • Direct antitumor effects (RNA nucleotide) specifically incubation with ATP breast and prostate cancer


  • Synergistic antitumor effects in conjunction with chemotherapy – in vitro studies suggest up to 90% dose reduction possible for certain cell types

  • IV ATP has halted progression of tumor cachexia (advanced cancer with rapid weight loss)

    50 mcg/kg/minute x 24 hours x 3 weeks

  • Protection of tissues from radiation injury

  • Increased natural killer cell function

  • Survival of patients in ICU for shock (multi-organ failure) increased from 70% to 100%


Current research chemotherapy – in vitro studies suggest up to 90% dose reduction possible for certain cell types

Enhanced

Nucleotide

Supplementation


Reducing Homocysteine reduces the chemotherapy – in vitro studies suggest up to 90% dose reduction possible for certain cell types

risk of death from the top 5 killer diseases:

Heart Attack by 80%

Strokes by 82%

Cancer by 33%

Diabetes by ??%

Alzheimer's by 50%


Homocysteine Levels chemotherapy – in vitro studies suggest up to 90% dose reduction possible for certain cell types (units = micromoles/litre)

Below 6.3 = Very Low Risk

6.4 - 9.0 = Low Risk

9.1 - 15.0 = High Risk

+ 15.0 = Very High Risk

Every 5 point decrease in homocysteine level =

50% reduced risk of cardiovascular death

26% reduced risk of cancer death


Methylation formula study dose response curve homocysteine level treatment group n 22
Methylation Formula Study Dose Response Curve chemotherapy – in vitro studies suggest up to 90% dose reduction possible for certain cell typesHomocysteine Level- Treatment Group (n=22)

Active Supplementation Group Months 0-3

11

10

9.2

9

8

7.1

7

6.8

P=.00001

6.1

6

P=.00001

P=.00001

0

1

2

3


Methylation formula study dose response curve homocysteine level placebo group n 11
Methylation Formula Study Dose Response Curve chemotherapy – in vitro studies suggest up to 90% dose reduction possible for certain cell typesHomocysteine Level- Placebo Group (n=11)

9

7.9

7.9

8

7.8

P= NS

P= NS

7.3

7

P= NS

6

0 1 2 3


Methylation chemotherapy – in vitro studies suggest up to 90% dose reduction possible for certain cell types Formula Study Dose Response CurveHomocysteine Level- Treatment Group for subjects with Baseline Homocysteine Level > 10 (n=7)

Active Supplementation Group Months 0-3

14

13.2

12

10

9.3

8.3

P=.009

8

7.3

P=.001

P=.00001

0

1

2

3


Population study completed in Western Norway with over 18,000 subjects – Hordaland Study

Directly established a relation between homocysteine levels and chronological aging

Homocysteine level of >13 was associated with someone 65 years old

Homocysteine level of <7 was associated with someone 25 years old


Bryan Maphalala - AIDS Victim 18,000 subjects – Hordaland Study

Normal

AIDS

Brian


Bryan Maphalala - AIDS Victim 18,000 subjects – Hordaland Study

Before taking CELLFOOD and CELLFOOD DNA-RNA CD4 Count: 10 units; Viral Load: 201,000

After taking CELLFOOD & CELLFOOD DNA-RNA for 6 months Mrs. Winnie Mandela congratulated him for his work with HIV and AIDS people


Does it really work

Does it really work? 18,000 subjects – Hordaland Study


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