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RB GUS P Ubi TEVL Xa3 NOS Hpt LB PowerPoint PPT Presentation


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BamH I. BamH I. A. Clone MKbF in vector pU1301. B. BamH I. BamH I. HindIII. -691. +37. RB P WRKY13 Xa3 NOS Hpt LB. Clone 12IMKb in vector pI1381. Dra I. Dra I. C. Clone MKb in vector pCAMBIA1301.

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RB GUS P Ubi TEVL Xa3 NOS Hpt LB

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Rb gus p ubi tevl xa3 nos hpt lb

BamHI

BamHI

A

Clone MKbF in vector pU1301

B

BamHI

BamHI

HindIII

-691

+37

RB PWRKY13 Xa3 NOS Hpt LB

Clone 12IMKb in vector pI1381

DraI

DraI

C

Clone MKb in vector pCAMBIA1301

RB GUSPUbi TEVL Xa3 NOS Hpt LB

RB GUSPXa3 Xa3 NOS Hpt LB

Supplemental Figure 1.Schematic diagrams of the transformation constructs. RB and LB, right and left T-DNA border; GUS, -glucuronidase gene; Hpt, hygromycin phosphotransferase gene; PUbi, maize ubiquitin gene promoter; TEVL, 5‘-nontranslated region of the tobacco etch virus; NOS, nopaline synthase polyadenylation signal. (A) overexpression construct of Xa3, MKbF, containing the complete genomic sequence of Xa3 coding region. (B) Pathogen-induced construct of Xa3, 12IMKb, containing the complete genomic sequence of Xa3 coding region. PWRKY13 is OsWRKY13 gene promoter locating at -691 to +37 of OsWRKY13 of rice variety Minghui 63. (C) Construct containing a 7.5-kb DNA fragment harboring Xa3 and its native promoter (PXa3) from Minghui 63.


Rb gus p ubi tevl xa3 nos hpt lb

10

Zhonghua 11 background

Minghua 63

9

8

MKbZH2 (PXa3:Xa3)

Zhonghua 11 (wild type)

7

MKbFZH1 (PUbi:Xa3)

6

5

4

3

2

Bacteria [log (cfu/leaf)]

Mudanjiang 8 background

10

Minghua 63

9

Mudanjiang 8 (wild type)

8

Rb17-2 (PXa3:Xa3)

7

MKbFMDJ2 (PUbi:Xa3)

6

5

4

3

0 6 12

2

Days after inoculation

Supplemental Figure 2.Growth of Xoo strain PXO61 in the leaves of rice plants at four-leaf stage. Indica rice variety Minghua 63 is the donor of Xa3. Japonica rice varieties Zhonghua 11 and Mudanjiang 8 are wild type plants. MKbZH2, MKbFZH1 and MKbFMDJ2 are resistant transgenic plants of T1 generation and Rb17-2 is homogeneous transgenic line. The bacterial population was determined from three leave at each time point by counting colony-forming units (cfu).


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