ChIP-on-Chip and Differential Location Analysis. Junguk Hur School of Informatics. October 4, 2005. Overview. Introduction to Transcriptional Regulation ChIP-on-Chip (ChIP-Chip) Current Approaches Our Approach. Transcription. Translation. Protein. RNA. The Central Dogma. DNA.
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School of Informatics
October 4, 2005
* If gene regulation goes awry? => Developmental abnormality
=> Diseases such as Chronic myeloid leukemia rheumatoid arthritis
DNA binding proteins
The amino acid – DNA recognition is not straightforward
Experiments can pinpoint binding sites on DNA
Leucine zipperTranscription Factor Binding Sites
Probabilistic model(profile of a binding site)
Modeling Binding Sites
Given a set of (aligned) binding sites …
Array of intergenic sequences from the whole genome
Whole-genome yeast intergenic microarray bound by Rap1
(A, B: binding ratio of TF in condition 1 and 2 respectively)
Any question and suggestion ?