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BRUCELLOSIS BURDEN IN BEEF ANIMALS AND RISK TO ABATTOIR WORKERS IN MASAKA DISTRICT , UGANDA, 2012

BRUCELLOSIS BURDEN IN BEEF ANIMALS AND RISK TO ABATTOIR WORKERS IN MASAKA DISTRICT , UGANDA, 2012. Henry Mulondo , F. Monge , D. Mugabi , G. Asiimwe, C. Odong , H. Kazoora T. Odoch , L. Mayega , M . Kirumira , I. Nabukenya Makerere University +256782165915 henrymulodo@gmail.com .

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BRUCELLOSIS BURDEN IN BEEF ANIMALS AND RISK TO ABATTOIR WORKERS IN MASAKA DISTRICT , UGANDA, 2012

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  1. BRUCELLOSIS BURDEN IN BEEF ANIMALS AND RISK TO ABATTOIR WORKERS IN MASAKA DISTRICT , UGANDA, 2012 Henry Mulondo , F. Monge, D. Mugabi, G. Asiimwe, C. Odong, H. Kazoora T. Odoch, L. Mayega, M. Kirumira , I. Nabukenya Makerere University+256782165915henrymulodo@gmail.com

  2. Outline • Background • Study objectives • Methodology • Results • Discussion • Conclusion and Recommendations • Public health actions taken • Acknowledgement

  3. Background • Brucellosis: zoonosis; affects animals and humans • Cause: bacteria of genus Brucella; has different species • B. abortus, B.melitensis, B. ovis, B. suis and B. canis • B. melitensis and B.suis from goats and pigs most virulent • Endemic in many countries especially those in Africa • Transmission: - Contact with body fluids, aborted fetuses, fetal membranes - Consumption of raw/poorly prepared animal products, - Inhalation of contaminated aerosols - Self inoculation.

  4. Backgroundcont’d • Worldwide, over 500,000 new cases are registered annually • Africa prevalence is 5-55% in humans and 8-46% in animals. • Uganda: 18-24% in humans,15.8% in cattle and 26% in goats • April 2012, an abattoir worker diagnosed with brucellosis • study was conducted May – July 2012

  5. Study objectives • Determine sero-prevalence of brucellosis - slaughtered beef animals - abattoir workers • Identify risks associated with brucellosis transmission to abattoir workers

  6. Methodology • Study design: cross sectional study • Study setting: Masaka Municipal abattoir - Abattoir lies in Katwe-Butego division - Slaughters 120-140 beef animals per week and has 30 workers • Study populations: - Beef animals Slaughtered from May-July 2012 - Workers in Masaka municipal abattoir • Sampling technique: - Abattoir workers self selected - beef animals selected by simple random method • Sample size: 24 abattoir workers and 236 beef animals • Inclusion criteria: - workers ≥18 years who verbally consented - beef animals slaughtered (May-July 2012)

  7. Methodology cont’d Map of Masaka

  8. Methodology cont’d • Blood samples from workers collected from brachial vein by nurse • Cattle samples collected from jugular vein by Principal investigator • Qualitative data was collected on; - socio-demographic characteristics of worker, breeds, sex mostly slaughtered - pregnancy status of females, protective gear use and duties done - Mini survey conducted, 24 abattoir workers interviewed - KI conducted with 4 Veterinary staff using KI guide • Testing serum samples: -Human samples; St. Agnes Lab using BMAT • Cattle samples; microbiology lab (COVAB)using Rose Bengal’s test • Data entered, cleaned and analyzed using Epinfo version 3.5.1

  9. Results Dependent variables • Analysis of blood samples showed that - abattoir workers: 6(25%) were Brucellosis +ve - titer values 160-320

  10. Result cont’dResults from the survey with abattoir workersIndependent variablesTable 1. Socio demographic characteristics of workers in Masaka municipal abattoir n=24 Variable Response Masaka municipal abattoir (n=24,%) Age (years) 20-25 2(8.3) 26- 30 10(41.7) >30 12(50.0) Religion Catholic 11(45.8) Muslims 7(29.2) Protestants 6(25.0) Marital Single 6(25.0) Status Maried18(75.0) Level of Primary 14(58.3) Education Secondary 4(16.7) Tertiary 1(4.2) university 5(20.8) work < 5 2(16.7) Experience (yrs) 5-10 8(33.3) > 10 12(50.0)

  11. Results cont’d • Breeds slaughtered: 21(87.5%) ankole, 2(8.3%) borans and 1(4.2%) sahiwal • Majority 224(95%) were females • Of the 224 females, 170(76%) were pregnant • Protective gear use: 23(95.8%) had partial set of protective gears (gumboots and overalls) • Only 1 (4.2%) had a full set (gumboots, overalls, gloves, face and nose masks)

  12. Results cont’dTable 2. Bivariate analysis to determine the association between duties done with Brucella infection , n= 24(fishers’ exact test used) Variable Response Total ill p-value Response Slaughter cattle Yes 16 04 0.586 No 08 02 Meat inspection Yes 02 00 0.586 No 22 06 Cleaning abattoir Yes 08 00 0.059 No 16 06 Handling of offals, Yes 04 04 0.001*** Fetuses, and fetal No 20 02 Remains Carrying carcasses Yes 01 01 0.250 No 23 05 Moving on track Yes 01 01 0.255 with beef cattle No 23 05 *** statistically significant factor

  13. Results cont’d Table 2 shows the association between use of personal protective gears with Brucellosis infection (fishers exact test used) Variable Response Total ill p-value Response Personal Full gear 01 00 0.750 Protective gear Partial gear 23 06

  14. Results cont’dResults from KI • All respondents (100%) reported that beef animals were from - Ssembabule, Lyaantode, Rakai and Gomba markets • More cows slaughtered than bulls because they were the most screened • of the 4 KI conducted, 75%(3) said majority of the cows were pregnant they look healthy, heavy and fetch more money • 50%(2) reported that the “high infection rate among offal, fetus and fetal remain handlers was due to high bacterial load on fetus and fetal remain” • All KI suggested that to prevent brucellosis in animals and humans - Sensitization of animal and meat handlers - Mass vaccination of healthy animals - Regular screening of animal and meat handlers

  15. Discussion • Sero-prevalence was high in beef cattle because; - animals were from pastoral communities that don’t vaccinate against brucellosis - Farmers culled and sold animals due to infertility • Sero-prevalence was high among abattoir workers handling offals fetuses and fetal remains due to - Erythritol produced by the placenta - the sugar stimulates multiplication of brucella organisms in reproductive systems of pregnant female animals to very high levels predisposing handlers to the infection • Most slaughtered animals were pregnant - pregnant animals look healthy, heavy and fetch more money • Bivariate analysis of risk factors identified - handling offals, fetuses and fetal remains as a risk

  16. Conclusions and Recommendations Conclusions: • Sero-prevalence of brucellosis in slaughtered beef cattle and abattoir workers was high • Handling offals, fetuses and fetal remains was a risk Recommendations: • Brucellosis prevention in humans depends on its control in animals • Routine mass vaccination of livestock animals should be done • Sensitization of animal and meat handlers including consumer about brucellosis • Regular screening of abattoir workers

  17. Public health actions taken • Findings were disseminated to participants and district stake holders • Infected workers were encouraged to seek treatment

  18. Acknowledgement • AFENET • RESPOND • MAAIF • Masaka District Local Government (MDLG) • COVAB • St Agnes Laboratory, NyendoMasaka

  19. THANK YOU

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