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Introduction to Gel Electrophoresis

Introduction to Gel Electrophoresis. Outline. How to prepare a gel How to micropipet Practice setting up electrophoresis Discussion viewing of gel In a later lab you will view and photograph your results. Agarose is weighed out. Agarose is diluted and boiled in buffer solution.

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Introduction to Gel Electrophoresis

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  1. Introduction to Gel Electrophoresis

  2. Outline • How to prepare a gel • How to micropipet • Practice setting up electrophoresis • Discussion viewing of gel • In a later lab you will view and photograph your results

  3. Agarose is weighed out

  4. Agarose is diluted and boiled in buffer solution

  5. Agarose solution is poured into gel holder This is a “comb”

  6. Agarose cools and solidifies Notice the “sample wells” comb

  7. Next: How to pipet into the sample well

  8. Select either a 100 or 200 ul micropipet from your lab station (1000 ul= 1 ml) Set at 25 ul Place on a yellow tip

  9. Push plunger to “first stop” Place tip in solution Aspirate sample by releasing plunger

  10. Carefully place the tip of the micropipet just inside the well Dispense solution by pushing to second stop Release tip by “ejection button”

  11. Gel and samples are subjected to electric current

  12. Samples migrate through the gel at different rates Negative electrode Positive electrode

  13. View your DNA samples with UV light Bio 22

  14. Photograph the results

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