1 / 16

Gel Electrophoresis

Gel Electrophoresis. By: Brian Barnes, Gary Pope, Eliza Morgan. What is GE?. Pronounced Gel ee-LEK-tro-fo-REE-sis Technique for separating DNA or Protein Molecules into strands according to length. What’s The Point?. Used to separate DNA and identify strands of interest

marcia-mcfarland
Download Presentation

Gel Electrophoresis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Gel Electrophoresis By: Brian Barnes, Gary Pope, Eliza Morgan

  2. What is GE? • Pronounced Gel ee-LEK-tro-fo-REE-sis • Technique for separating DNA or Protein Molecules into strands according to length

  3. What’s The Point? • Used to separate DNA and identify strands of interest • Strands of interest are further analyzed once identified

  4. The Process • Test tube contains DNA • Samples put in different “wells” • Each well contains unique enzyme

  5. The Process • Gel Matrix, usually agarose • Blue tracking dye added for identification purposes • Electric current applied to move negatively charged DNA through agarose

  6. The Process • Agarose like a spongy barrier • Shorter strands of DNA move more freely through the gel • Longer strands have more difficulty moving

  7. The Process • Blue Dye is visible moving through gel • As dye touches the end, current is turned off

  8. The Process • Fluorescent dye is added to identify molecules • Size Standards Well contains known DNA fragments to compare sizes • Measured by Base Pairs (bp)

  9. The Process • Each band is comprised of millions of fragments of the same size

  10. Analysis • Now to isolate fragments of interest… • The Blot- a copy of the results which enables the researcher to identify fragments of interest

  11. Analysis • Gel is placed in basic solution • DNA denatures into single strands rather than double helix

  12. Analysis • Transferred to salt solution • Nylon Filter placed on top • Absorbent paper towels placed on filter

  13. Analysis • Salt solution drawn upward by paper towels • DNA adheres to nylon filter • Filter is treated, DNA adheres permanently

  14. Analysis • Radioactive DNA “probe” added • DNA probe complimentary to band of interest • Probe hybridizes with bands of interest

  15. Analysis • Filter washed to remove un-hybridized DNA • X-Ray film placed over filter • Radioactive probe exposed under X-Ray

  16. Using the Analysis • Now the strands of interest are identified • Identical gel is made • DNA of interest is cut out for further analysis

More Related