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DNA Fingerprinting Supplies from Iowa State Student Preparation DNA and Enzyme Preparation

Obtaining Supplies(Iowa Teachers Only)Teachers should request the following supplies (next slide) at least a week in advance. Supplies should be ordered by contacting Lori Miller by phone in Iowa, 1/800-643-9504 or 515/294-9818, or by Fax: 515/294-4629. The electrophoresis equipment can be o

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DNA Fingerprinting Supplies from Iowa State Student Preparation DNA and Enzyme Preparation

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    5. Preparation of the Student Materials The supplies can best be provided to the class in groups of five students. The DNA samples should be refrigerated until class time. It is too expensive for the Office of Biotechnology to provide DNA for every student in a class. The office will provide enough DNA, restriction endonuclease, and reaction buffer for a minimum of two groups of five students or a maximum of one group of five students in every class section, whichever is greater.

    6. DNA Fingerprinting Preparation of the Student Materials (cont.) For the other student groups, use distilled water to replace the DNA, restriction endonuclease, and reaction buffer. Every group of students should be provided with the blue migration dye.

    8. DNA and Enzyme Prep Step 1 For each group of students label 7-1.5 ml tubes "C,1,2,3,4, N and D" with a permanent felt pen. Tube "N" will contain the enzyme mixture. Tube "D" will contain the migration dye.

    9. DNA and Enzyme Prep Step 2 Pipette 17 µl of a 0.025 µg/µl concentration of pBR322 DNA into the tube labeled "C."

    10. DNA and Enzyme Prep Step 3 In the tube labeled "1" pipette 17 µl of 0.15 µg/µl concentration of lambda DNA.

    11. DNA and Enzyme Prep Step 4 In the tube labeled "2" pipette 17 µl of 0.075 µg/µl concentration of Ad-2 DNA.

    12. DNA and Enzyme Prep Step 5 In the tube labeled "3" pipette 17 µl of 0.025 mg/ml concentration of pBR322 DNA.

    13. DNA and Enzyme Prep Step 6 In the tube labeled "4" pipette 17 µl of 0.025 µg/µl concentration of pUC19 DNA.

    14. DNA and Enzyme Prep Step 7 In the microcentrifuge tube (1.5 ml) labeled "N," pipette 15 µl of reaction buffer and 3 µl Bgl 1.

    15. DNA and Enzyme Prep Step 8 In a microcentrifuge tube (1.5 ml) labeled "D," pipette 40 µl of blue migration dye.

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