cord blood derived regulatory t cells a novel cure for graft versus host disease
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Cord Blood-Derived Regulatory T Cells: A Novel Cure for Graft-Versus-Host-Disease. Siddharth Damania 2007. University of Minnesota Cancer Center. Bone-Marrow and Stem-Cell Transplants. 6,000 Americans. http://www.vacationusarentals.com/images/usa_map.gif.

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cord blood derived regulatory t cells a novel cure for graft versus host disease

Cord Blood-Derived Regulatory T Cells:A Novel Cure for Graft-Versus-Host-Disease

Siddharth Damania

2007

bone marrow and stem cell transplants
Bone-Marrow and Stem-Cell Transplants

6,000 Americans

http://www.vacationusarentals.com/images/usa_map.gif

bone marrow and stem cell transplants1
Bone-Marrow and Stem-Cell Transplants

http://www.southalabama.edu/nursing/MSN%20Info/surgery_nurse.jpg

bone marrow and stem cell transplants2
Bone-Marrow and Stem-Cell Transplants

Over half develop GVHD

http://www.southalabama.edu/nursing/MSN%20Info/surgery_nurse.jpg

graft versus host disease gvhd
Graft-Versus-Host-Disease (GVHD)
  • GVHD occurs when transplanted cells reject host cells in the patient

Transplanted

Cell

Patient Cell

purpose
Purpose
  • To use T-reg cells to cure mice with human GVHD

GVHD

GVHD

GVHD

GVHD

GVHD

first goal
First Goal
  • To devise a new protocol to derive and purify CD4+25+ T-reg cells using human umbilical cord blood
types of t cells
Types of T cells
  • Cytotoxic T cells
  • Helper T cells
  • T-reg cells

http://www.britannica.com/eb/art/print?id=85110&articleTypeId=0

cd4 25 t reg cells
CD4+25+ T-reg Cells
  • Sakaguchi (2005)
    • Increased capability to suppress immune responses
    • Low numbers increase risk of autoimmune diseases
cd4 25 t reg cells1
CD4+25+ T-reg Cells
  • Sakaguchi (2005)
    • Increased capability to suppress immune responses
    • Low numbers increase risk of autoimmune diseases
  • Suri-Payer et al (2006)
    • Potential to suppress multiple types of autoimmune diseases
umbilical cord blood
Umbilical Cord Blood
  • Godfrey et al (2005)
    • T-reg cells isolated from peripheral blood are often impure and require multiple purification processes
umbilical cord blood1
Umbilical Cord Blood
  • Godfrey et al (2005)
    • T-reg cells isolated from peripheral blood are often impure and require multiple purification processes
    • Umbilical cord blood facilitates easier purification and presents purer quantities of T-reg cells
procedure4
Procedure

CD4+25+T-reg Cells

second goal
Second Goal
  • To culture derived CD4+25+ T-reg cells
second goal1
Second Goal
  • To culture derived CD4+25+ T-reg cells

CD4+25+T-reg Cells

second goal2
Second Goal
  • To culture derived CD4+25+ T-reg cells

CD4+25+T-reg Cells

second goal3
Second Goal
  • To culture derived CD4+25+ T-reg cells

CD4+25+T-reg Cells

CD4+25+T-reg Cells

third goal
Third Goal
  • To analyze the histological effect of CD4+25+ T-reg cells in GVHD mice
procedure7
Procedure

GVHD

GVHD

GVHD

GVHD

GVHD

procedure8
Procedure

GVHD

GVHD

GVHD

GVHD

GVHD

procedure9
Procedure

GVHD

GVHD

GVHD

GVHD

GVHD

future work
Future Work
  • Optimize culture expansion
future work1
Future Work
  • Optimize culture expansion
  • Increase rate of cure in mice
future work2
Future Work
  • Optimize culture expansion
  • Increase rate of cure in mice
  • Test T-reg cells as a therapeutic cure for GVHD in humans
acknowledgements
Acknowledgements
  • Dr. Keli Hippen
  • Dr. Paul Harker-Murray
  • The University of Minnesota
  • Lois Fruen
  • Advanced Science Research
cord blood derived regulatory t cells a novel cure for graft versus host disease1

Cord Blood-Derived Regulatory T Cells:A Novel Cure for Graft-Versus-Host-Disease

Siddharth Damania

2007

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