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Structural Studies on Single Particles and Biomolecules

Structural Studies on Single Particles and Biomolecules. Janos Hajdu, Uppsala University Gyula Faigel, Institute for Solid State Physics and Optics, Budapest Keith Hodgson, Stanford Synchrotron Radiation Laboratory Chris Jacobsen, State University of New York at Stony Brook

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Structural Studies on Single Particles and Biomolecules

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  1. Structural Studies on Single Particles and Biomolecules Janos Hajdu, Uppsala University Gyula Faigel, Institute for Solid State Physics and Optics, Budapest Keith Hodgson, Stanford Synchrotron Radiation Laboratory Chris Jacobsen, State University of New York at Stony Brook Janos Kirz, State University of New York at Stony Brook Gerd Materlik, HASYLAB, DESY, Hamburg John Miao, Stanford Synchrotron Radiation Laboratory Richard Neutze, Uppsala University Carol V. Robinson, New Chemistry Laboratory, Oxford University David Sayre, State University of New York at Stony Brook Abraham Szöke, Lawrence Livermore National Laboratory Edgar Weckert, HASYLAB, DESY, Hamburg David van der Spoel, Uppsala University

  2. ~300 nm Ø (cell membrane: 8 nm) Solvent content: 60-70% Mycoplasmas MYCOPLASMAS The smallest creatures capable of self-replication 1 DNA (genome size: 600 - 1,300 kbp) 400 ribosomes 10,000 RNA molecules 50,000 protein molecules 400,000,000 water/solute molecules

  3. Structural Biology: The Damage Problem • Biological samples are highly radiation sensitive • Conventional methods cannot achieve atomic resolution on non- repetitive (or non-reproducible) structures • The limit to damage tolerance is about 200 X-ray photons/Å2 • in crystals (conventional experiments) • The conventional damage barrier can be stretched by very • fast imaging (Neutze, R., Wouts, R., van der Spoel, D., Weckert, E. Hajdu, J. (2000) Nature 406, 752-757)

  4. Scattering by a Crystal and by a Single Molecule PROTEIN CRYSTAL SINGLE MACROMOLECULE Planar section, simulated image Max. resolution is a function of crystal quality Max. resolution does not depend on sample quality

  5. (1) Photoelectric effect (~90%) followed by Auger emission, shake-up excitations, and interactions between decay channels (2) Elastic scattering (~7-10%) (3) Inelastic scattering (~3%) Scattering and Damage by X-rays (biological samples: C,N,O,H,S) LCLS - a “never seen regime”, for which only predictions and simulations exist The LCLSBeam Interacts with the Matter Through Scattering and Absorption:

  6. LCLS Coulomb Explosion of Lysozyme (50 fs) Radiation damage interferes with atomic positions and the atomic scattering factors

  7. Modelling Sample Dynamics XMD interfaced with GROMACS(van der Spoel et al.) Heatingconserving momentum Bond breakthrough Morse potential Ionisationprimary and secondary effects Ionisation dynamicscalculate changes in the elastic, inelastic and photoelectric cross-sections for each atom during exposure Inventorykept on all electrons in the sample (Neutze, R., Wouts, R., van der Spoel, D., Weckert, E. Hajdu, J. (2000) Nature 406, 752-757)

  8. Ionisation and Coulomb Explosion of a Protein Molecule (Lysozyme) in Intense X-ray Pulses 3x1012 photons/100 nm diameter spot (3.8x106 photons/Å2, 12 keV) Ionisation events Energy

  9. Landscape of Damage Tolerance Ionisation and subsequent sample explosion causes diffraction intensities to change Agreement factor: Photons/pulse/100 nm spot Time (fs)

  10. Sample Size and Scattering HRV ~3,000,000 Da LYSOZYME 19,806 Da RUBISCO 562,000 Da Structure of content unknown

  11. P u ls e du ra t i o n ( F W HM ) 10 f s 50 f s 10 0 f s 23 0 f s P h ot o ns / pu l s e ( 10 0 n m sp o t ) 12 11 11 10 5 x 10 8 x 10 3 x 10 5 x 10 (R = 1 5%) S i ng l e l yso z y m e mo l ec u l e 26 Å 30 Å >30 Å >30 Å M W : 19 , 80 6 3 x 3x 3 cl us t e r o f l yso z ym e s <2 . 0 Å 3 . 0 Å 6 . 5 Å 12 Å T ot al M W : 53 5 ,0 0 0 S i ng l e RUB ISCO mo l ec u le 2 . 6 Å 4 . 0 Å 20 Å 30 Å M W : 56 2 ,0 0 0 S i ng l e v i r al ca psid ( TBS V ) <2 . 0 Å <2 M W : ~ 3 ,0 0 0 , 000 Calculated Limits of Resolution with Relectronic = 15 % . 0 Å <2 . 0 Å 2 . 4 Å Single virus particles look very promising

  12. First Experiments • Single viral particles - structure of the viral genome • Nanoclusters • Structural kinetics on nanometer-sized samples • Nanocrystals • Two dimensional crystalline arrays • X-ray diffraction tomography of whole cells • X-ray scattering from intact cells

  13. Sample Handling 1.Spraying Techniques Sample selection and injection Nanodroplets, Cryogenic Temperatures, High Vacuum • Native proteins, • Viruses, • Nanoclusters, • Nanocrystals, • Cell organelles, • Intact cells 2. Sample embedded in vitreous ice Goniostat, Cryogenic Temperatures, High Vacuum • Intact cells, cell organelles

  14. 100% 50% 0% 10000 15000 20000 25000 30000 (m/z) Prototype High Mass Q-TOF Mass Spectrometer COLLECTOR PROBE PUSHER LOW FREQUENCY QUADRUPOLE MASS FILTER EXTRACTION LENS DETECTOR SAMPLE GRID RF HEXAPOLE RF HEXAPOLE SAMPLE INJECTOR FIRST MASS MEASUREMENT: MS2 virus: 2,484,700 Da TIME-OF-FLIGHT ANALYSER REFLECTRON (Tito et al. (2000) J. Am. Chem. Soc. 122, 3550-3551)

  15. Interaction Chamber and Detector Arrangement SAMPLE INJECTION X-RAY PULSE INTELLIGENT BACK-STOP DIAGNOSTICS and LASER PORT DETECTOR 2 DETECTOR 1 To MASS/CHARGE ANALYSIS and ELECTRON MICROSCOPY

  16. A Planar Section across the Molecular Transform of TBSV 2 Å resolution

  17. A Planar Section across the Molecular Transform of TBSV 8 Å resolution

  18. The Phase Problem • Continuous molecular transforms (oversampling) • Tools of classical crystallography (these should work with reproducible structures) • Holography

  19. How is Nucleic Acid Organised inside a Virus? Artist’s view: Nucleic acid is released at the right time in the right order Courtesy of D. I. Stuart, Oxford Experiment: Gouet et al. Cell 97, 481-490 (1999)

  20. Structural Kinetics on Nanocrystals and Nanoclusters Most biochemical processes involve diffusion of reactants Kinetic studies in crystals suffer from “the mixing problem” Decorated nanoclusters may help to overcome this limitation Implications for Functional Genomics

  21. ~300 nm Ø (cell membrane: 8 nm) Solvent content: 60-70% Mycoplasmas MYCOPLASMAS The smallest creatures capable of self-replication 1 DNA (genome size: 600 - 1,300 kbp) 400 ribosomes 10,000 RNA molecules 50,000 protein molecules 400,000,000 water/solute molecules

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