1 / 15

Chromatography

Chromatography. Partition chromatography. redistribution (partition) of the molecules between the stationary and mobile phase. Plate model of chromatography of mixture of compounds partitioning 1:1 (bold) and 1:3 (italics) between the mobile and stationary phase. .

trygg
Download Presentation

Chromatography

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Chromatography Biophysical Tools '04 - Chromatography

  2. Partition chromatography • redistribution (partition) of the molecules between the stationary and mobile phase Plate model of chromatography of mixture of compounds partitioning 1:1 (bold) and 1:3 (italics) between the mobile and stationary phase. • larger number of plates – better separation Biophysical Tools '04 - Chromatography

  3. Adsorption • stationary phases: alumina, activated carbon, hydroxyapatite Column Support Biophysical Tools '04 - Chromatography

  4. TLC and GLC Thin layer chromatography Gas-liquid chromatography Biophysical Tools '04 - Chromatography

  5. Kinds of chromatography Biophysical Tools '04 - Chromatography

  6. Gel exclusion Biophysical Tools '04 - Chromatography

  7. Molecular Weight by Gel Exclusion Biophysical Tools '04 - Chromatography

  8. rate column height Optimization Biophysical Tools '04 - Chromatography

  9. Sample size Biophysical Tools '04 - Chromatography

  10. Ion-Exchange chromatography Biophysical Tools '04 - Chromatography

  11. Elution gradient salt gradients or pH Biophysical Tools '04 - Chromatography

  12. pH of the elution Biophysical Tools '04 - Chromatography

  13. HPLC • Extremely large number of plates  high pressure rate of elution Biophysical Tools '04 - Chromatography

  14. Other types of chromatography: • Affinity (couple ligand to CNBr-activated Sepharose) • Hydrophobic (low pressure RP-HPLC equivalent) Biophysical Tools '04 - Chromatography

  15. Filtration and Dialysis Dialysis change buffer often • Filtration • nitrocellulose & fiberglass • Centricon series Reverse dialysis Biophysical Tools '04 - Chromatography

More Related