1 / 15

Data Blitz

Data Blitz. Eric Spivey 26 March 2010. Biopolymer Solution. Background: Using pulsed laser-generated multiphoton excitation to fabricate micron scale hydrogel matrices from biopolymer solutions. Biopolymer Hydrogel Matrix. Focused, pulsed laser. Microscope Objective.

tova
Download Presentation

Data Blitz

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Data Blitz Eric Spivey 26 March 2010

  2. Biopolymer Solution Background: Using pulsed laser-generated multiphoton excitation to fabricate micron scale hydrogel matrices from biopolymer solutions. Biopolymer Hydrogel Matrix Focused, pulsed laser Microscope Objective Motivation: Use this method to fabricate cell scaffolds for neural tissue engineering applications. Goal:Fabricate hydrogel microstructures with tunable functional and mechanical properties.

  3. Hertz Model Force = F Radius R Displacement = d • Can calculate Young’s Modulus with F, R and d, • Assume: • Poisson’s ratio is 0.5 and • Substrate measured is infinitely thick • Corrections exist for non-infinite substrates

  4. Moduli of Multiphoton-fabricated structures 4800 ± 430 Pa 3900 ± 220 Pa

  5. Force-indentation

  6. Bead-tipped Cantilevers

  7. Using high-k Cantilever

  8. Using high-k Cantilever

  9. Dwell time measurements

  10. Dwell time measurements

  11. Next • More dwell time experiments with different structure types and lower k cantilevers • Validate system using PDMS standard • Functionalization – continue work with diffusible gradients in flow chamber • CNT – effect of CNT on modulus of GMHA/BSA hybrid structures.

  12. The basketball team from my undergraduate institution is superior to the basketball team from your undergraduate institution

  13. Fabricated Microstructures 5 µm 5 µm

  14. Other

  15. Repeatability 80 µm/sec 4 mW 1 mW 2mW 3 mW Day 1 Day 3

More Related