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Data Blitz

Data Blitz. Eric Spivey 5 May 2010. Biopolymer Solution. Background: Using pulsed laser-generated multiphoton excitation to fabricate micron scale hydrogel matrices from biopolymer solutions. Biopolymer Hydrogel Matrix. Focused, pulsed laser. Microscope Objective.

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Data Blitz

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  1. Data Blitz Eric Spivey 5 May 2010

  2. Biopolymer Solution Background: Using pulsed laser-generated multiphoton excitation to fabricate micron scale hydrogel matrices from biopolymer solutions. Biopolymer Hydrogel Matrix Focused, pulsed laser Microscope Objective Motivation: Use this method to fabricate cell scaffolds for neural tissue engineering applications. Goal:Fabricate hydrogel microstructures with tunable functional and mechanical properties.

  3. Hertz Model Force = F Radius R Displacement = d • Can calculate Young’s Modulus with F, R and d, • Assume: • Poisson’s ratio is 0.5 and • Substrate measured is infinitely thick • Corrections exist for non-infinite substrates

  4. Force-indentation

  5. Dwell time measurements

  6. Dwell Time Measurements

  7. Dwell Time Measurements

  8. Finite Element Modeling From Dintwa et. al. Granular Matter. 2008

  9. Implications

  10. Protein Cantilevers From Khirpin et. al. Soft Matter. 2010 (in press)

  11. BSA/GMHA Cantilevers

  12. Next • Validate system using PDMS standard – initial attempts with high modulus sample did not work • Use lower force, lower strain system (low spring constant, large R) to take measurements (reduce high strain systemic error) • Incorporate high-strain systemic error estimation into current model • Further explore “protein cantilever” method

  13. Fabricated Microstructures 5 µm 5 µm

  14. Other

  15. Repeatability 80 µm/sec 4 mW 1 mW 2mW 3 mW Day 1 Day 3

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