1 / 10

Effects on lipid levels and lipid metabolism under more human-like conditions.

Aim of the study: Effects of garlic on lipid metabolism and atherosclerosis. Effects on lipid levels and lipid metabolism under more human-like conditions. Lipid levels, cholesterol synthesis and cholesterol absorption.

tillmans
Download Presentation

Effects on lipid levels and lipid metabolism under more human-like conditions.

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Aim of the study: Effects of garlic on lipid metabolism and atherosclerosis • Effects on lipid levels and lipid metabolism under more human-like conditions. • Lipid levels, cholesterol synthesis and cholesterol absorption. • Effects on fast atherosclerosis development and other processes that might be related with atherosclerosis. • Inflammation, blood pressure, platelet activation and atherosclerosis analysis.

  2. APOE*3-Leiden transgenic mouse as a model for hyperlipidemia and atherosclerosis studies • Transgenic mice carrying a mutant form of human APOE*3 gene. • Dominant dysfunctional APOE*3-Leiden results in defect in chylomicron/ VLDL clearance and more human-like lipoprotein profile. • Highly responsive to feeding cholesterol-rich diets. • Develops atherosclerosis varying from fatty streaks to more human-like complicated lesions.

  3. Effect of garlic on lipid metabolism and atherosclerosis in APOE*3-Leiden mice: Selection of compounds • Lichtwer LI111 garlic powder: Reference dry garlic powder. • Morado de Cuenca 200 (Mor200): Garlic powder rich in organic sulfur-containing compounds. • Diallyl disulfide (DADS): Isolated garlic compound rich in sulfur. • Allicin: Reference isolated garlic compound reported to result in 43% reduction of fatty streaks in C57Black/6 mice (D. Abramovitz et al., Coronary Artery Disease, 1999, 10: 515-519)

  4. 80 Effect of garlic on lipid metabolism and atherosclerosis in APOE*3-Leiden mice: Experimental scheme 25 -2 0 Regular diet Western diet Atherogenic diet 6 14 Run in 8 10 12 16 18 20 22 2 4 Matching and start of the study 80 65 Weight and food intake 80 80 80 65 60 65 65 65 Blood sampling/ plasma isolation 80 80 65 65 65 65 65 60 80 Plasma lipid levels 80 65 80 65 65 65 65 Cholesterol lipoprotein profile p p p p p p Chol. synthesis/ Intestinal absorption p p p p p Marker for liver function p p p Liver lipid composition 15 60 Blood pressure 80 65 65 Platelet activation measurements 80 65 Inflammation markers 60 Atherosclerotic aorta lesions typing 60 5 Atherosclerotic aorta lesions area 60 5 Compound biostability p p p p p Inflammation and atherosclerosis related parameters General parameters Lipid metabolism related parameters

  5. Plasma cholesterol levels Regular diet Western diet Atherogenic diet mM Control 3.1 9.3 63 LI111 2.9 9.3 57 * Mor 200 3.0 9.1 44 DADS 2.9 8.3 70 * Allicin 3.5 9.2 73 Effect of garlic on plasma cholesterol levels under 3 dietary conditions *P< 0.05

  6. squalene 1.5 lathosterol lanosterol 1.0 0.5 Concentration (µmol sterol / mmol cholesterol) 0.0 Control LI111 Mor 200 DADS Allicin 40 campesterol b-sitosterol 30 20 10 0 Control LI111 Mor 200 DADS Allicin Effect of garlic on markers of cholesterol synthesis and intestinal absorption under 3 dietary conditions Regular diet Markers of cholesterol synthesis Markers of intestinal absorption *P< 0.05

  7. Effect of garlic on liver function under 3 dietary conditions Plasma alanine amino transferase Regular diet Western diet Atherogenic diet U/L Control 33.4 30.3 175 t LI111 104 33.2 34.4 t Mor 200 94.1 32.9 30.3 DADS 29.9 41.8 203 Allicin 25.2 28.2 171 tP=0.051

  8. 8 250 p=0.001 p<0.001 200 6 * 150 * SAA (µg/ ml) vWF (%PP) 4 100 2 50 0 0 LI111 Mor 200 DADS LI111 Mor 200 DADS Allicin Control Allicin Control Effect of garlic on Serum Amyloid A and von Willebrand factor concentrations under fast atherogenic conditions *P< 0.05

  9. Effect of garlic powders on atherosclerosis under more human-like conditions: Groups selection • Control • Primador FRANCE 2001: 0.64 g/kg b.w. of dry garlic powder with low sulphur content. • Primador FRANCE 2001: 6.4 g/kg b.w. of dry garlic powder with high sulphur content. • Kyolic (positive control): 200 mg/kg b.w. of powder rich in S-allylcysteine reported to result in 64% reduction of fatty streaks in New Zealand white rabbits. Campbell JH, Efendy JL, et al. J.Nutrition. 2001;131:1006S-1009S.

  10. Effect of garlic powders on atherosclerosis under more human-like conditions: Experimental design Diet T + 0.2% cholesterol 28 In weeks 0 Run in 26 4 8 12 16 20 24 Matching and start of the study 60 Weight and food intake 60 60 60 55 60 60 60 60 Blood sampling/ plasma isolation 60 60 60 60 60 60 60 55 Plasma lipid levels 60 60 60 60 60 60 60 p Cholesterol lipoprotein profile p p Marker for liver function p p p p p p p p SAA and Fibrinogen 55 60 60 vWf, t-PA and soluble ICAM 55 60 60 All blood stimulation assay 55 Aortic root lesions typing and area 5 55 Aortic arch area and lipids deposition 55 5 Aortic root lesions composition 55 5 Mice on standard chow diet 8 5 Liver lipid composition (p.m.) 55 Compound biostability p p Inflammation and atherosclerosis development related parameters General parameters Lipid metabolism related parameters

More Related