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% positive cells

A. B. D. C. 20. 20. 10. % Apoptotic nuclei. % positive cells. 10. **. 0. 0. PM. DM. PM/IR. PM. DM. PM/IR.

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% positive cells

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  1. A B D C 20 20 10 % Apoptotic nuclei % positive cells 10 ** 0 0 PM DM PM/IR PM DM PM/IR Effect of localized X-irradiation on astrocytic differentiation of NSCs. A, Immunofluorescent detection of DSBs visualized 53BP1 foci (red). White circle indicates the targeted area. B, Higher magnification picture indicates localized DSBs within the targeted area. The nuclei are counter stained with DAPI (Blue). C, Astrocytic differentiation of NSCs determined by anti-GFAP antibody. D, Neural differentiation of NSCs determined by anti-b-III tubulin antibody. IR; localized X-irradiation. The data represent the mean ± SEM. **p < 0.01. Bar indicates 50 µm. Figure S5

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