200

1. 200 200 160 160 120 120 80 80 40 40 0 0 100 100 101 101 102 102 103 103 104 104 γδ T cells A B αβ T cells Counts CD25 Supplemental figure 1 Distinct stimulation status of αβ and γδ T cells induced by CD28 and cross-bound CD3 mAbs. Magnetically isolated αβ (A) and γδ (B) T cells were cultured in the abscence (black line) or presence (grey line) of CD28 and 10 µg/ml cross-linked plate-bound anti-CD3 mAb for 72h. Activation status was determined by CD25 expression measured by flow cytometry as described in Materials and Methods.

2. 100 100 80 80 60 60 40 40 20 20 0 0 104 104 102 102 100 100 101 101 103 103 + anti-TLR-2 + anti-TLR-4 A B Counts FasL Supplemental figure 2 E. coli Nissle 1917 did not increase FasL expression on stimulated γδ T cells cultured with anti-TLR-2 and anti-TLR-4 mAbs. Magnetically isolated γδ T cells were stimulated with IPP and IL-2 and cultured with blocking anti-TLR-2 (A) or anti-TLR-4 (B) mAbs. γδ T cells were incubated in the absence (grey line) or presence (black line) of 25% (vol/vol) E. coli Nissle 1917-CM. FasL expression was determined by flow cytometry as described in Material and Methods. The graphs represent typical donors and are representative of three individual experiments.