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Objective

Induction and Maintenance of HIV-1-Specific Immune Responses in Exposed Sero -negative (ESN) Women of Sero -discordant Couples from Southern India.

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Objective

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  1. Induction and Maintenance of HIV-1-Specific Immune Responses in Exposed Sero-negative (ESN) Women of Sero-discordant Couples from Southern India Suniti Solomon1, K.G. Murugavel1, R.Vignesh1, D. Bella1, R. Shoba1, S. Poongulali1, N. Kumarasamy1, A. Landay2, L. Baum2, Sunil S. Solomon1,3, C.R. Swathirajan 1, P. Balakrishnan1, Susan Cu-Uvin4, K.H. Mayer5, B.L. Shacklett6 1YRG CARE, Chennai, India; 2Rush University, Chicago, IL, USA; 3Johns Hopkins School of Medicine, MD, USA; 4Brown University, Providence, RI, USA; 6Beth Israel Deaconess Hospital, MA, USA; 6UC Davis, Davis, CA, USA.

  2. Objective • To determine the breadth and persistence of HIV-1 specific cytotoxic T lymphocyte (CTL) responses in blood and cervical mucosa of ESN women. Study Population • 30 ESN women provided samples at baseline and at least one follow up visit (3rd ,6th month). • Sero-concordant couples (n=17) and low-risk seronegative women (n=30) provided samples only at baseline and were included as controls. Methods • Peripheral blood mononuclear cells (PBMC) and mucosal mononuclear cells (MMC) were stimulated with HIV-1 Gag and Env (Clade C) and IFN-g production was measured by Intracellular cytokine staining (ICS) assay. • The flow data were analyzed using FlowJo software.

  3. Results Conclusion • These findings confirm that HIV-specific T-cell responses can be detected in PBMC and MMC from ESN women. • In ESN women, the PBMC responses were exclusively directed against Env. • One out of 4 ESN women continued to show positive responses upon longitudinal follow-up.

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