1 / 43

Cell Biology I. Overview II. Membranes: How Matter Get in and Out of Cells

Cell Biology I. Overview II. Membranes: How Matter Get in and Out of Cells III. DNA, RNA, and Chromosome Structure IV. Protein Synthesis V. Origin of Life Hypotheses. Cell Biology I. Overview II. Membranes: How Matter Get in and Out of Cells III. DNA, RNA, and Chromosome Structure

sook
Download Presentation

Cell Biology I. Overview II. Membranes: How Matter Get in and Out of Cells

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Cell Biology I. Overview II. Membranes: How Matter Get in and Out of Cells III. DNA, RNA, and Chromosome Structure IV. Protein Synthesis V. Origin of Life Hypotheses

  2. Cell Biology I. Overview II. Membranes: How Matter Get in and Out of Cells III. DNA, RNA, and Chromosome Structure IV. Protein Synthesis V. Origin of Life Hypotheses Origin of life hypotheses Evolutionary Theory

  3. A. The Early Earth and Earth History 4.5 bya: Earth Forms Graviational sorting of materials…heavy to the core, gases released under pressure…

  4. The Early Earth and Earth History • - Earliest Atmosphere - probably of volcanic origin Gases produced were probably similar to those released by modern volcanoes (H2O, CO2, SO2, CO, S2, Cl2, N2, H2) and NH3 and CH4

  5. A. The Early Earth and Earth History 4.0 bya: Oldest Rocks 4.5 bya: Earth Forms

  6. A. The Early Earth and Earth History 4.0 bya: Oldest Rocks 3.4 bya: Oldest Fossils 4.5 bya: Earth Forms Stromatolites - communities of layered 'bacteria'

  7. A. The Early Earth and Earth History 4.0 bya: Oldest Rocks 3.4 bya: Oldest Fossils 4.5 bya: Earth Forms Putative microfossil bacteria from Australia that date to 3.4 by

  8. A. The Early Earth and Earth History 2.3-2.0 bya: Oxygen in Atmosphere 4.0 bya: Oldest Rocks 3.4 bya: Oldest Fossils 4.5 bya: Earth Forms

  9. A. The Early Earth and Earth History 2.3-2.0 bya: Oxygen 1.8 bya: first eukaryote 4.0 bya: Oldest Rocks 3.4 bya: Oldest Fossils 4.5 bya: Earth Forms

  10. A. The Early Earth and Earth History 2.3-2.0 bya: Oxygen 0.9 bya: first animals 1.8 bya: first eukaryote 4.0 bya: Oldest Rocks 3.4 bya: Oldest Fossils 4.5 bya: Earth Forms

  11. A. The Early Earth and Earth History 2.3-2.0 bya: Oxygen 0.9 bya: first animals 1.8 bya: first eukaryote 0.5 bya: Cambrian 4.0 bya: Oldest Rocks 3.4 bya: Oldest Fossils 4.5 bya: Earth Forms

  12. A. The Early Earth and Earth History 2.3-2.0 bya: Oxygen 0.9 bya: first animals 1.8 bya: first eukaryote 0.5 bya: Cambrian 0.24 bya:Mesozoic 4.0 bya: Oldest Rocks 3.4 bya: Oldest Fossils 4.5 bya: Earth Forms

  13. A. The Early Earth and Earth History 2.3-2.0 bya: Oxygen 0.9 bya: first animals 1.8 bya: first eukaryote 0.5 bya: Cambrian 0.24 bya:Mesozoic 0.065 bya:Cenozoic 4.0 bya: Oldest Rocks 3.4 bya: Oldest Fossils 4.5 bya: Earth Forms

  14. A. The Early Earth and Earth History 4.5 million to present (1/1000th of earth history) 2.3-2.0 bya: Oxygen 0.9 bya: first animals 1.8 bya: first eukaryote 0.5 bya: Cambrian 0.24 bya:Mesozoic 0.065 bya:Cenozoic 4.0 bya: Oldest Rocks 3.4 bya: Oldest Fossils 4.5 bya: Earth Forms

  15. B. The Formation of Biologically Important Molecules - Oparin-Haldane Hypothesis (1924): - in a reducing atmosphere, biomonomers would form spontaneously Aleksandr Oparin (1894-1980) J.B.S. Haldane (1892-1964)

  16. B. The Formation of Biologically Important Molecules - Oparin-Haldane Hypothesis (1924): - in a reducing atmosphere, biomonomers would form spontaneously - Miller-Urey (1953) all biologically important monomers have been produced by these experiments, even while changing gas composition and energy sources

  17. B. The Formation of Biologically Important Molecules - Oparin-Haldane Hypothesis (1924): - in a reducing atmosphere, biomonomers would form spontaneously - Miller-Urey (1953) - Sydney Fox - 1970 - polymerized protein microspheres

  18. B. The Formation of Biologically Important Molecules - Oparin-Haldane Hypothesis (1924): - in a reducing atmosphere, biomonomers would form spontaneously - Miller-Urey (1953) - Sydney Fox - 1970 - polymerized protein microspheres - Cairns-Smith (1960-70) - clays as templates for non-random polymerization - 1969 - Murcheson meteorite - amino acids present; some not found on Earth. To date, 74 meteoric AA's. - 2004 - Szostak - clays could catalyze formation of RNA's

  19. C. Acquiring the Characteristics of Life Three Primary Attributes: - Barrier (phospholipid membrane) - Metabolism (reaction pathways) - Genetic System

  20. C. Acquiring the Characteristics of Life 1. Evolution of a Membrane - form spontaneously in aqueous solutions

  21. C. Acquiring the Characteristics of Life • Evolution of a Membrane • Metabolic Pathways • - problem: • how can pathways with useless intermediates evolve? These represent 'maladaptive valleys', don't they? A B C D E How do you get from A to E, if B, C, and D are non-functional?

  22. C. Acquiring the Characteristics of Life • Evolution of a Membrane • Metabolic Pathways • - Solution - reverse evolution A B C D E

  23. C. Acquiring the Characteristics of Life • Evolution of a Membrane • Metabolic Pathways • - Solution - reverse evolution suppose E is a useful molecule, initially available in the env. E

  24. C. Acquiring the Characteristics of Life • Evolution of a Membrane • Metabolic Pathways • - Solution - reverse evolution suppose E is a useful molecule, initially available in the env. As protocells gobble it up, the concentration drops. E

  25. C. Acquiring the Characteristics of Life • Evolution of a Membrane • Metabolic Pathways • - Solution - reverse evolution Anything that can absorb something else (D) and MAKE E is at a selective advantage... D E

  26. C. Acquiring the Characteristics of Life • Evolution of a Membrane • Metabolic Pathways • - Solution - reverse evolution Anything that can absorb something else (D) and MAKE E is at a selective advantage... but over time, D may drop in concentration... D E

  27. C. Acquiring the Characteristics of Life • Evolution of a Membrane • Metabolic Pathways • - Solution - reverse evolution So, anything that can absorb C and then make D and E will be selected for... C D E

  28. C. Acquiring the Characteristics of Life • Evolution of a Membrane • Metabolic Pathways • - Solution - reverse evolution A B C D E and so on until a complete pathway evolves.

  29. C. Acquiring the Characteristics of Life • Evolution of a Membrane • Metabolic Pathways • Evolution of a Genetic System • - conundrum... which came first, DNA or the proteins they encode? DNA RNA (m, r, t) protein

  30. C. Acquiring the Characteristics of Life • Evolution of a Membrane • Metabolic Pathways • Evolution of a Genetic System • - conundrum... which came first, DNA or the proteins they encode? DNA DNA stores info, but proteins are the metabolic catalysts... RNA (m, r, t) protein

  31. C. Acquiring the Characteristics of Life • Evolution of a Membrane • Metabolic Pathways • Evolution of a Genetic System • - conundrum... which came first, DNA or the proteins they encode? • - Ribozymes • info storage AND • cataylic ability

  32. C. Acquiring the Characteristics of Life • Evolution of a Membrane • Metabolic Pathways • Evolution of a Genetic System • - conundrum... which came first, DNA or the proteins they encode? • - Ribozymes • - Self replicating molecules • - three stage hypothesis

  33. Stage 1: Self-replicating RNA evolves RNA

  34. Stage 1: Self-replicating RNA evolves RNA m- , r- , and t- RNA PROTEINS (REPLICATION ENZYMES) Stage 2: RNA molecules interact to produce proteins... if these proteins assist replication (enzymes), then THIS RNA will have a selective (replication/reproductive) advantage... chemical selection.

  35. DNA Reverse transcriptases m- , r- , and t- RNA PROTEINS (REPLICATION ENZYMES) Stage 3: Mutations create new proteins that read RNA and make DNA; existing replication enzymes replicate the DNA and transcribe RNA.

  36. Can this happen? Are their organisms that read RNA and make DNA?

  37. Can this happen? Are their organisms that read RNA and make DNA? yes - retroviruses....

  38. DNA m- , r- , and t- RNA Already have enzymes that can make RNA... PROTEINS (REPLICATION ENZYMES) Stage 3: Mutations create new proteins that read RNA and make DNA; existing replication enzymes replicate the DNA and transcribe RNA.

  39. DNA m- , r- , and t- RNA Already have enzymes that can make RNA... PROTEINS (REPLICATION ENZYMES) Stage 3: Mutations create new proteins that read RNA and make DNA; existing replication enzymes replicate the DNA and transcribe RNA.

  40. This is adaptive because the two-step process is more productive, and DNA is more stable (less prone to mutation). DNA m- , r- , and t- RNA PROTEINS (REPLICATION ENZYMES) Stage 4: Mutations create new proteins that replicate the DNA instead of replicating the RNA...

  41. This is adaptive because the two-step process is more productive, and DNA is more stable (less prone to mutation). DNA And that's the system we have today.... m- , r- , and t- RNA PROTEINS (REPLICATION ENZYMES) Stage 4: Mutations create new proteins that replicate the DNA instead of replicating the RNA...

  42. D. Summary: STEPS REQUIRED FOR THE SPONTANEOUS, NATURAL FORMATION OF LIFE, and the evidence to date: 1. Spontaneous synthesis of biomolecules - strong evidence; Miller-Urey experiments. 2. Polymerization of monomers into polymers (proteins, RNA, sugars, fats, etc.) - strong evidence;  Fox and Cairns-Smith experiments. 3. Formation of membranes - strong evidence; behavior of phospholipids in solution. 4. Evolution of metabolic systems - reasonable hypotheses, and genetic similarity in genes involved in particular pathways (suggesting gene duplication and subsequent evolution of new genes and elaboration of existing pathways) 5. Evolution of a genetic system - a reasonable hypothesis and significant corroborating evidence that it could happen. But no experimental evidence of the process evolving through all three steps. 6. How did these three elements (membrane, metabolism, genetic system come together?) a few untested hypotheses.

More Related