EXPERIMENTAL STUDIES

1. EXPERIMENTAL STUDIES

2. Epidemiological Studies • Epidemiological studies are either observational or experimental. • Observational studies are considered “natural” experiments • Experimental studies are considered true experiments 

3. Assigning Exposure • Defining feature – Investigator assigns exposure to study subjects • most closely resemble controlled laboratory experiments • serve as models for the conduct of observational studies • gold standard of epidemiological research • have high status and validity • can pick up small and modest effects

4. 2. Overall Conduct • Hypothesis formed • Study subjects recruited based on specific criteria • Informed consent • Eligible and willing subjects randomly allocated to receive treatment arm(s) • Study groups monitored for outcome • recurrence of disease • first occurrence of disease • getting better, side effects • Outcome rates compared across groups

5. 3. Ways to Categorize Experimental Studies • Individual versus community – treatment allocated to individual OR entire community • Do women with stage I breast cancer given a lumpectomy alone survive as long without recurrence of disease as women given a lumpectomy plus radiation? • Does fluoride in the water supply decrease the frequency of dental caries in a community compared to a similar community without such water treatment?

6. 3. Ways to Categorize Experimental Studies • Preventive versus therapeutic – prophylactic agent given to healthy or high-risk individual to prevent disease OR treatment given to diseased individual to reduce risk of recurrence, improve survival • Does tamoxifen lower the incidence of breast cancer in women with high risk profile compared to high risk women not given tamoxifen? • Do combinations of two or three antiretroviral drugs prolong survival of AIDS patients as well as regimens of single drugs? • Ethics – which study is easier to get approval for?

7. 4. Selection of Study Population A) Reference population - general group to whom results of a trial should be applicable (all humans, or some restrictions may apply) B) Study or experimental population – people who are considered for enrollment in a trial, potential participants Is this important – does it really matter who participates?

9. 5. Issues to be Considered • Size, size, size - not just number of people in the trial, but how many endpoints (outcome under study) are expected • Restrictions on who is eligible (eligibility criteria) • Substantive: What group are you interested in? • Logistics: What group is accessible? Who will comply with study protocol? How feasible is complete and accurate follow-up on the subjects? • Characteristics of volunteers: How does study population differ from total experimental population?

10. 6. Allocation of Treatment • Should be random assignment • DEFINITION: Each individual has the same chance of receiving each possible “treatment” • Some examples of random allocation • Random number table: as each subject enrolled, assigned a number from the random number table; assign even numbers to treatment A and odd to treatment B. • Toss a coin for each subject: heads=A, tails=B • Some examples of nonrandom allocation • Alternate assignment of treatments • Assignment by day of the week • Why are these not random?

11. Goal of Randomization • To achieve baseline comparability between compared groups on factors related to outcome • Known factors • Unknown factors • Essence of good comparison between “treatments” is that the compared groups are the same EXCEPT for the “treatment.”

12. Goal of Randomization • The compared groups should have the same distribution of all of these characteristics. • Equal distribution of known and unknown factors that are relevant to response to the treatment (confounders) • Larger study – improves randomization works • Do you ever have to worry about confounders?

13. 7. Use of Placebo and Blinding: Goals • Placebos are used to make the groups as comparable as possible • Blinding: • Single blind -- subjects do not know if they are receiving treatment or placebo • Double blind -- neither subjects investigators or coordinators know who is receiving treatment or placebo • Purpose of blinding: To avoid bias in ascertainment of outcome • Placebo allows study to be blind • Why don’t we always blind?

14. Maintenance/assessment of compliance A) Study requires active participation and cooperation of participants but deviations from the protocol will occur related to side effects, illness, level of interest, and length of follow-up B) Noncompliance makes the compared groups more alike • Why is this a problem? • Should you move a non-compliant person to the control arm? • Should you move someone who starts the intervention on their own in the control arm to the treatment arm? • Does this happen often?

15. Maintenance/assessment of compliance • Design phase strategy • Pick an interested group and design a simple protocol. • Problem with this? • Throughout study • frequent contact with subjects • incentives to continue • such as free check-ups • compensation for their time

16. 9. Ascertaining the Outcome A) Goals • High follow-up rates: don’t lose people • Why? • Uniform follow-up for compared groups: must be equally vigilant in follow-up in all compared groups • Why?

17. 10. Analysis of Data from Experimental Studies • Data set up: familiar 2 x 2 table • Measure of treatment effect: RR or RD

18. 11. Important Issues in Experimental Studies: Ethical Considerations • Equipoise • Must be genuine doubt about efficacy of treatment yet sufficient belief that it may work • Stopping rules • What if it becomes apparent, before the trial is over, that the new treatment is beneficial (and should not be withheld from the placebo group) or is toxic (and treatment should be withdrawn)?

19. 11. Important Issues in Experimental Studies: Planning for an informative result • If the study finds no difference between compared treatments, do you believe it? • Or was there a difference but the study was not powerful enough to detect it? • Type II Error – failed to find a true difference • Initial consideration is study size.

20. 11. Important Issues in Experimental Studies: Analyzing by intention to treat • As the saying goes… once randomized, always analyzed. • Why?

21. PRACTICE FOR THE EXAM

22. Analysis of Cohort Studies • Example: Tuberculosis treatment and breast cancer study • Followed 1,047 women who were treated with air collapse therapy and exposed to numerous fluoroscopic examinations (radiation) and 717 who received other treatments. • A total of 47,036 woman-years of follow-up were accumulated during which 56 breast cancer cases occurred.

23. Analysis of Cohort Studies • IR exposed = • IR unexposed = • RR = • Interpretation:

24. Find out RR, AR in the exposed and Population AR

25. Calculate the AR Proportion in the Exposed, and the Population AR Proportion

26. Example of age adjustment What is the crude death rate from stroke in Community X and in Community Y? Show your work. Using the provided standard population, what is the age adjusted death rate in community X and community Y.

27. Recently, there have been a number of norovirus outbreaks on cruise ships. The cruise ship owners contacted the CDC to conduct an in-depth analysis of the possible modes of transmission of norovirus in the cruise ship environment. CDC investigators interviewed all of the passengers on the last affected cruise (N=5700) and obtained information on the passenger’s recreational activities. They found the following results: 1,200 passengers developed gastroenteritis during the cruise. 3300 passengers never swam in the pool during the cruise. 800 passengers swam in the pool and developed gastroenteritis during the cruise. FYI: The cruise lasted one week. Set up a two by two table and calculate the risk ratio.