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CaM-Seph. CaM-Seph. + Suramin. + Suramin. Seph. Seph. Input. Figure 1. A. IP 3 R1. B. EGTA. Ca 2+. IP 3 R1 1-225. EGTA. Ca 2+. Figure 2. A. P 49-81. B. P 106-128. a b c d e f g h. a b c d e f g h. EGTA. Ca 2+. 0. 12.5.

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CaM-Seph

CaM-Seph

+ Suramin

+ Suramin

Seph

Seph

Input

Figure 1

A

IP3R1

B

EGTA

Ca 2+

IP3R1 1-225

EGTA

Ca 2+


Figure 2

A

P 49-81

B

P 106-128

a b c d e f g h

a b c d e f g h

EGTA

Ca2+

0

12.5

25

37.5

50

75

100

0

12.5

25

37.5

50

75

100

[Suramin] (µM)

[Suramin] (µM)

C

D


Figure 3

1499

1649

100 % 1-5-8-14

76 % IQ

60 % IQ

72% 1-5-8-14

A

1554-ldsqvnnlflkshnivqkta-1573

1554-ldsqvnnlflkshnivqktalnwrlsarnaar-1585

1564-kshnivqktalnwrlsarnaar-1585

1564-kshnivqktalnwrlsarnaarrdsvlaasrd-1595

B

C

a

b

c

d

e

a

b

c

d

e

Ca2+

EGTA

D

CaM

P1554-1573

P1554-1585

P1564-1585

P1564-1595


0

12.5

25

50

75

100

[Suramin] (µM)

Figure 4

A

a b c d e f g

EGTA

Ca2+

B


Figure 5

A

Lvec

L15

B

C



Figure 7

A

Lbs1

Lbs1 Δ1-225

*

control

suramin

control

suramin

B

IP3R1 Δ1-225

IP3R1

*

control

suramin

control

suramin



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