1 / 4

Proteomic, M etabolomic and L ipidomic Research Capability at ICH and ION

UCL. Proteomic, M etabolomic and L ipidomic Research Capability at ICH and ION. Lipidomics. UCL. Proteomic, M etabolomic and L ipidomic Research Capability at ICH and ION. Same principle as the proteomic mass spectral method

shad-mendez
Download Presentation

Proteomic, M etabolomic and L ipidomic Research Capability at ICH and ION

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. UCL Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION Lipidomics

  2. UCL Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION • Same principle as the proteomic mass spectral method • Urine/plasma/CSF injected into a UPLC QTOF mass spectrometer •  lipids separated by UPC2 chromatography (supercritical fluid chromatography) •  Uses CO2 to separate lipids out according to their polar properties not non-polar (reverse phase) •  Lipids are easier to identify than metabolites as they have been more extensively studied C22 C20 C18 C24 C16 C14 C12 Free fatty acids C10 C8

  3. UCL Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION • Same principle as the proteomic mass spectral method • Urine/plasma/CSF injected into a UPLC QTOF mass spectrometer •  lipids separated by UPC2 chromatography (supercritical fluid chromatography) •  Uses CO2 to separate lipids out according to their polar properties not non-polar (reverse phase) •  Lipids are easier to identify than metabolites as they have been more extensively studied Triacylglycerols Chosteryl esters ( Mixture of FFA, TAG, and CE

  4. UCL C34:0 Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION • Phospholipid analyses from human heart C32:0 Phosphatidylcholine C36:0 C30:1 C36:1 Phosphatidylethanolamine Phosphatidylcholine Sphingomyelin C36:3 C38:3 ceramides Lyso-phosphatidyl- ethanolamine Lysophosphatidyl choline Phosphatidylglycerol

More Related