'Whole genome shotgun sequencing' presentation slideshows

CS 394C Algorithms for Computational Biology

640 views • 47 slides

DNA Sequencing

DNA Sequencing. Method to sequence longer regions. genomic segment. cut many times at random ( Shotgun ). Get one or two reads from each segment. ~500 bp. ~500 bp. Reconstructing the Sequence (Fragment Assembly). reads. Cover region with ~7-fold redundancy (7X).

582 views • 46 slides

The Human Genome Race

The Human Genome Race. Collins vs. Venter. Collins Venter. Collins. Francis Collins, a physician, is director of the National Human Genome Research Institute.

639 views • 42 slides

Mapping and Sequencing Genomes

Mapping and Sequencing Genomes. Sanger Sequencing. Sanger Sequencing. Sanger Sequencing-Critical Innovations. Lee Hood (1986) Radiolabelled ddNTPs ?Fluorescently Labelled ddNTPs eliminate radioactivity, multiplex reactions 2) Molly Craxton (1991)

1.09k views • 41 slides

Genome Assembly: a brief introduction

593 views • 35 slides

WGS Assembly and Reads Clustering

WGS Assembly and Reads Clustering. Zemin Ning. Production Software Group Informatics Division. Outline of the Talk:. Whole Genome Shotgun Sequencing Insert Sizes Repeats in the Genomes Kmer Words Hashing and Distribution Relational Matrix Profile of unique kmer words Phusion Steps

314 views • 23 slides

Hierarchical Sequencing

Hierarchical Sequencing. a BAC clone. map. Hierarchical Sequencing Strategy. Obtain a large collection of BAC clones Map them onto the genome (Physical Mapping) Select a minimum tiling path Sequence each clone in the path with shotgun Assemble Put everything together. genome.

346 views • 19 slides