'Shotgun sequencing' presentation slideshows

Lecture 6 Some useful bits & pieces that every Perl programmer should know

Lecture 6 Some useful bits & pieces that every Perl programmer should know. Strictness. Use strict;

1.04k views • 92 slides

Genomic Sequencing

Genomic Sequencing. DNA Sequencing. Fred Sanger, Cambridge, England Copy DNA with one of four bases starved ACGTAAGCTA with T starved produces ACG and ACGTAAGC Run experiment with each of four bases starved, producing a ladder (all sub-fragments ending at the base)

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Why so hard for Classical Marine Biologists and Microbiologists?

The Sorcerer II Global Ocean Sampling Expedition: Metagenomic Characterization of Viruses within Aquatic Microbial Samples

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Sequencing a genome

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Mohamed Tikah Marrakchi BIN6002 Summer, 2005

Mohamed Tikah Marrakchi BIN6002 Summer, 2005. An Eulerian path approach to DNA fragment assembly Pavel A.Pevzner, Haixu Tang, and Michael S. Waterman PNAS 2001. Genome sequencing. Analysing DNA using improved technologies opens a new era in (but not only in) biomedical research.

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Genomics

Genomics. DNA array s a nd chips (semi) qRT- PCR Northern blot + hybrid. Trans k rip tional fusions. 2D ele c tro phoresis Mass spectrometry Protein sequencing Translational fusional Immunodetection Enzyme activities. Regula tion. Chromatogra phy Mass spectrometry NMR.

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Venter et al.

Community structure and metabolism through reconstruction of microbial genomes from the environment articles. Venter et al. Outline. Addressing : uncultivated microbial communities with unclear roles in natural system Source of Study: Acid Mine Drainage (AMD) microbial biofilm.

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Linkage Disequilibrium-Based Single Individual Genotyping from Low-Coverage Short Sequencing Reads

Linkage Disequilibrium-Based Single Individual Genotyping from Low-Coverage Short Sequencing Reads. Justin Kennedy 1 Joint work with Sanjiv Dinakar 1 , Yozen Hernandez 2 , Ion Mandoiu 1 , and Yufeng Wu 1 1 CSE Department, University of Connecticut

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Next Generation Sequencing Data Analysis

Next Generation Sequencing Data Analysis. Nadia Pisanti, University of Pisa. Why sequencing?. The knowledge of DNA and RNA sequences has become a crucial tool for: Basic research in biology, pharmacology and medicine.

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GENOMA HUMANO, 26 Junho 2000

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559 views • 27 slides

Perl Grabbag

Some useful bits'n'pieces that every Perl programmer should know. Perl Grabbag. Strictness. #! /usr/bin/perl -w # bestrict - demonstrating the effect of strictness. use strict; $message = "This is the message. "; print $message;. Results from bstrict.

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Genomic DNA Cloning

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THE GENOME

THE GENOME. DESCRIBE THE MAIN FEATURES OF THE HUMAN GENOME INCLUDING DETAILS OF AT LEAST ONE SPECIFIC CHROMOSOME ELAINE WHITFIELD. THE HUMAN GENOME. The human genome actually consists of two genomes: - complex nuclear genome - simple mitochondrial genome. NUCLEAR

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Biological Motivation for Fragment Assembly

Biological Motivation for Fragment Assembly. Rhys Price Jones Anne R. Haake. What is fragment assembly?. The reconstruction of the contiguous chromosomal DNA sequence from short, experimentally-generated fragments.

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C A M E R A A Metagenomics Resource for Marine Microbial Ecology

C A M E R A A Metagenomics Resource for Marine Microbial Ecology. July 27, 2007 Paul Gilna UCSD/Calit2 Saul A. Kravitz J. Craig Venter Institute. Acknowledgements. UCSD/Calit2 Larry Smarr, PI; Paul Gilna, Executive Director Phil Papadopoulos, Technical Lead Weizhong Li JCVI

529 views • 19 slides

DNA Sequencing

DNA Sequencing. Kabi R. Neupane, Ph.D. Leeward Community College ABE Workshop 2006. What to Sequence?. The RT-PCR product has been inserted into the pCR4-TOPO vector Our goal -Sequence the insert DNA. We Supplied. Template DNA: Your plasmid Primer DNA: T3 or T7. Frederick Sanger.

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Genome sequencing and assembling

Genome sequencing and assembling. Chitta Baral. Basic ideas and limitations. Current lab techniques can sequence small (say 700 base pairs) DNA pieces. Use restriction enzymes to cut DNA pieces Sort pieces of different sizes using gel electrophoresis and use the sorting to read them

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Chromosome 12

Chromosome 12.

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