'Primer extension' presentation slideshows

The Puzzling Properties of Peptidyl Transferase

The Puzzling Properties of Peptidyl Transferase. Gregory W. Muth Department of Chemistry St. Olaf College. Peptidyl Transferase Reaction. Composition of the Ribosome. Proposed General Acid-Base Mechanism of Peptidyl Transferase. General Acid Catalysis. General Base Catalysis.

843 views • 47 slides

Measuring gene expression via RNA

Northern blot RNase protection Primer extension RT-PCR Q-RT-PCR Microarray RNAseq. Measuring gene expression via RNA. Northern blotting. Denaturing gel for true MW (urea, formamide). Alternative polyadenylation sites ? 2 dhfr mRNAs. http://www.gene-quantification.de/mrna.html#northern.

690 views • 45 slides

Molecular SEQUENCE DATA Generation - From template DNA to Sequence Alignment or other stuff

Molecular SEQUENCE DATA Generation - From template DNA to Sequence Alignment or other stuff. Protocol. Collect tissue samples from whatever and extract tDNA Amplify specific gene using PCR (Polymerase Chain Reaction) Sequence PCR products Align our sequence with published sequences

632 views • 42 slides

Lipase: protein that hydrolyses lipids Polymerase: protein that builds polymers

Naming enzymes. Lipase: protein that hydrolyses lipids Polymerase: protein that builds polymers Ligase: protein that ligates DNA fragments Proteinase or protease: protein that hydrolyses proteins DNase: protein that hydrolyses DNA RNase: protein that hydrolyses RNA.

650 views • 40 slides

Chapter 7 Analyzing DNA and gene structure, variation and expression

Chapter 7 Analyzing DNA and gene structure, variation and expression. Sequencing and genotyping DNA Standard/manual DNA sequencing using dideoxynucleotide chain terminators ddATP, ddGTP, ddCTP, and ddTTP. . 07_01.jpg. 07_02.jpg. 07_02_2.jpg.

471 views • 31 slides

Abdel-Rahman et al. PNAS 98: 2538 (2001)

Caused by defects in repair of chromosome breaks and defects in the DNA damage checkpoint. Genome instability in tumor cells Truncations Translocations Inversions Duplications Amplifications Deletions Mutations. Abdel-Rahman et al. PNAS 98: 2538 (2001).

460 views • 31 slides

Methods in Molecular Biology

Molecular Biology Bio4751 Spring 2003 Gary A. Bulla, PhD. Methods in Molecular Biology. RNA quantitation. a. Northern. 1. Lyse with detergent, 2. Phenol extract (removes proteins) 3. Precipitate RNA 4. Load onto agarose gel 5. Transfer RNA to nylon membrane

830 views • 29 slides

174 views • 17 slides

MIT Molecular Machines ( Jacobson ) Group jacobson@media.mit.edu 3.9.13

Fabricational Complexity. MIT Molecular Machines ( Jacobson ) Group jacobson@media.mit.edu 3.9.13. What Drives T he C ost of Placing A toms W here W e W ant T hem ? What are The F undamental L imits ?. Si Wafer with Area sufficient for 2 Billion Transistors Cost: ~$0.50.

300 views • 16 slides

Polymerase Chain Reaction: A Markov Process Approach

Polymerase Chain Reaction: A Markov Process Approach. Mikhail V. Velikanov et al. J. theor. Biol. 1999 Summarized by ??? 2003.8.12. Contents. Introduction Markov Process Model for Primer Extension Model for Multi-Cycle PCR Runs Numerical Results Discussion. Introduction.

272 views • 14 slides

Haplotypes determination and new variants within the ovine prion CDS region.

240 views • 14 slides

Accessing Genetic Variation: Genotyping Single Nucleotide Polymorphisms

291 views • 14 slides

Polymerase Chain Reaction: A Markov Process Approach

Polymerase Chain Reaction: A Markov Process Approach. Mikhail V. Velikanov et al. J. theor. Biol. 1999 Summarized by ??? 2003.8.12. Contents. Introduction Markov Process Model for Primer Extension Model for Multi-Cycle PCR Runs Numerical Results Discussion. Introduction.

143 views • 14 slides

Wang Group

158 views • 4 slides

Additonal file 1

Additonal file 1. Supplementary Figure S1. Scheme of inverse PCR and recovery of the origin sequence

116 views • 2 slides

orf6 - complementation test Wild type + pNBV1 Wild type + pNBV1 + orf6 gene

orf6 - complementation test Wild type + pNBV1 Wild type + pNBV1 + orf6 gene orf6 - mutant + pNBV1 orf6 - mutant + orf6 gene Complementation test ?? (SMB-glucose, SMB-CO) Primer extension orf1-6 , cutR , cutB ? ?? primer extension ? RT-PCR ?? FixAB cloning

141 views • 2 slides