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Genotypic Microbiological Methods

Genotypic Microbiological Methods

Genotypic Microbiological Methods. Can be used to determine genetic composition of organisms: Identify organisms (diagnostics) Identify distinct groups of organisms (taxonomy/systematics) by examining similarity between organisms Examine temporal or spatial variation of populations

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476 views • 29 slides
Analysis of Microbial Community Structure

Analysis of Microbial Community Structure

Analysis of Microbial Community Structure. Historical Perspective on Microbial Diversity Assessing Diversity Alone Diversity With Phylogeny Diversity of Function. Historical Perspective on Microbial Diversity. Knowledge is limited by our tools.

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713 views • 13 slides
Analysis of Microbial Community Structure

Analysis of Microbial Community Structure

Analysis of Microbial Community Structure. Historical Perspective on Microbial Diversity Assessing Diversity Alone Diversity With Phylogeny Diversity of Function. Historical Perspective on Microbial Diversity. Knowledge is limited by our tools.

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247 views • 13 slides
-- 1353/1078/872 -- 603 -- 310 -- 281/271 -- 234 -- 194 -- 118 -- 72

-- 1353/1078/872 -- 603 -- 310 -- 281/271 -- 234 -- 194 -- 118 -- 72

Supplemental Figure 1 A and B: Specificity validation of the PCR products Supplemental Figure 1A: Agarose gel electrophoresis of qPCR amplicons Gel strength: 4% NuSieve GTG (FMC Bioproducts, Rockland, ME) low melting temperature agarose

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163 views • 2 slides
Overview: Human Alu insertion polymorphism lab steps

Overview: Human Alu insertion polymorphism lab steps

Overview: Human Alu insertion polymorphism lab steps. 1. Cheek cell (Method A) or hair sheath cell (Method B) sampling - saline (0.9% NaCl) mouth wash and cell centrifugation (cheek cells) - add protease solution (hair follicle cells) - add Chelex solution (cheek cells)

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237 views • 1 slides
Overview: Human Alu insertion polymorphism lab steps

Overview: Human Alu insertion polymorphism lab steps

Overview: Human Alu insertion polymorphism lab steps. 1. Cheek cell (Method A) or hair sheath cell (Method B) sampling - saline (0.9% NaCl) mouth wash and cell centrifugation (cheek cells) - add protease solution (hair follicle cells) - add Chelex solution (cheek cells)

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143 views • 1 slides

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