'Cdna' presentation slideshows

Functional Genomics

2.08k views • 112 slides

RT-PCR Obtain the internal region of cDNA

RT-PCR Obtain the internal region of cDNA 1. Mix crude RNA or poly(A) RNA, cDNA primer (one of the amplification primers in 7.) 2. Heat at 65 0 C for 3-15 min. 3. Cool on ice 4. Add RT and RT buffer, 42 0 C (37 0 C to 55 0 C, depending on the composition of primer and RNA) for 1 hr.

757 views • 54 slides

Microarray

Microarray. Simultaneously determining the abundance of multiple(100s-10,000s) transcripts. Gene expression assays. DNA microarrays rely on the hybridization properties of nucleic acids to monitor transcript or RNA abundance on a genome-wide scale in different types of cells.

487 views • 33 slides

Microarray

Microarray. Simultaneously determining the abundance of multiple(100s-10,000s) transcripts. Gene expression assays. DNA microarrays rely on the hybridization properties of nucleic acids to monitor transcript or RNA abundance on a genome-wide scale in different types of cells.

373 views • 33 slides

Recombinant DNA II

Recombinant DNA II. Making, screening and analyzing cDNA clones Genomic DNA clones. cDNA clones are copies of mRNAs. Much of the genomic DNA is not expressed as mRNA Many issues about gene function are best addressed by examining the product that they encode.

459 views • 23 slides

MCB 7200: Molecular Biology

MCB 7200: Molecular Biology. Gene libraries cDNA libraries Library screening. Eukaryotic gene organization. enhancers silencers. Genomic library construction.

203 views • 20 slides

MCB 720: Molecular Biology

MCB 720: Molecular Biology. Gene libraries cDNA libraries Library screening. Eukaryotic gene organization. enhancers silencers. Genomic library construction.

361 views • 19 slides

MCB 720: Molecular Biology

MCB 720: Molecular Biology. Gene libraries cDNA libraries Library screening. Eukaryotic gene organization. enhancers silencers. Genomic library construction.

198 views • 19 slides

Biotechnology and Genetic Engineering PBIO 4500/5500

Biotechnology and Genetic Engineering PBIO 4500/5500. Gene libraries cDNA libraries Library screening. Eukaryotic gene organization. enhancers silencers. Genomic library construction.

190 views • 18 slides

biomed.miami/arrays/images/agilent_array.jpg

Example of a DNA array used to study gene expression (note green, yellow red colors; also note that only part of the total array is depicted). http://www.biomed.miami.edu/arrays/images/agilent_array.jpg. DNA Arrays: Two Purposes. Genotype individuals on polymorphisms (read text for example).

251 views • 16 slides

biomed.miami/arrays/images/agilent_array.jpg

Example of a DNA Array (note green, yellow red colors; also note that only part of the total array is depicted). http://www.biomed.miami.edu/arrays/images/agilent_array.jpg. Methods:.

153 views • 15 slides

DNA Microarray:

504 views • 13 slides

Lab meeting 2013.05.13

291 views • 10 slides

Differential Sperm Cell Gene Expression in Plumbago

Differential Sperm Cell Gene Expression in Plumbago.

86 views • 1 slides

Molecular Negative 0.01 0.1 1 10 pg cDNA

A. Molecular Negative 0.01 0.1 1 10 pg cDNA Marker control 0.5 0.5 0.5 0.5 pg IS. 1018bp. Log (ActRI/Internal Standard). -1 0 1. 652bp. 492bp. 506bp.

77 views • 1 slides

Methodology

Methodology. U937 Human Immune Cells. Estrogen (5 uM). Control (No treatment). 4-nonylphenol (5 uM). Cultured Cells, RNA Isolation, RT to cDNA. Applied cDNA to microarray chips Scanned chips for detection of gene expression by chemiluminescence. Data analyzed by Spotfire software.

84 views • 1 slides

A Novel Approach to Eliminate Vector Background and Increase Sequencing Efficiency of cDNA.

Introduction. Abstract. Conclusion. MuA. cDNA insert. cDNA insert. pCMVSport6/ Gateway Expression clone. Gateway Entry clone. att L1. att L2. att B1. att B2. vector. vector. Experimental details. Materials. Results and Discussion. Mechanism. pDONR vector. att P1. att P2.

111 views • 1 slides