Biochemical instrumental analysis-2. Dr. Maha Al- Sedik. Atomic absorption spectrophotometer. Atomic absorption spectrophotometer. Atomic absorption spectroscopy is a quantitative method of analysis that is applicable to many metals such as Ca , Fe , Al and copper.
Dr. Maha Al-Sedik
In AAS, the sample is atomised – ieconverted into free atoms in the vapourstate.
Process of converting an analyte in solid, liquid or solution form to a free gaseous atom.
The greater the number of atoms in the vapor, the more radiation is absorbed.
hollow cathode lamp
hollow cathode lamp for Aluminum (Al) is shown below
The cathode lamps are stored in a compartment inside the AA spectrometer. The specific lamp needed for a given metal analysis is rotated into position for a specific experiment.
Differences between atomic absorption spectrophotometer and the flameless atomic absorption spectrophotometer: