Biochemical instrumental analysis-2. Dr. Maha Al- Sedik. Atomic absorption spectrophotometer. Atomic absorption spectrophotometer. Atomic absorption spectroscopy is a quantitative method of analysis that is applicable to many metals such as Ca , Fe , Al and copper.
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Biochemical instrumental analysis-2
Dr. Maha Al-Sedik
Atomic absorption spectrophotometer
In AAS, the sample is atomised – ieconverted into free atoms in the vapourstate.
The Atomisation
Process of converting an analyte in solid, liquid or solution form to a free gaseous atom.
Components
Components :
The light source:
hollow cathode lamp
hollow cathode lamp for Aluminum (Al) is shown below
The Manganese Flame
The Potassium Flame
The Copper Flame
The Calcium Flame
photomultiplier tube
http://www.youtube.com/watch?v=_KZjb9G3hB8irst.pptx
http://www.youtube.com/watch?v=HBegTB_WDxQ
Flameless atomic absorption spectrophotometry
Differences between atomic absorption spectrophotometer and the flameless atomic absorption spectrophotometer: