Biochemical instrumental analysis-2. Dr. Maha Al- Sedik. Atomic absorption spectrophotometer. Atomic absorption spectrophotometer. Atomic absorption spectroscopy is a quantitative method of analysis that is applicable to many metals such as Ca , Fe , Al and copper.
Biochemical instrumental analysis-2
Dr. Maha Al-Sedik
Atomic absorption spectrophotometer
In AAS, the sample is atomised – ieconverted into free atoms in the vapourstate.
Process of converting an analyte in solid, liquid or solution form to a free gaseous atom.
The light source:
hollow cathode lamp
hollow cathode lamp for Aluminum (Al) is shown below
The Manganese Flame
The Potassium Flame
The Copper Flame
The Calcium Flame
Flameless atomic absorption spectrophotometry
Differences between atomic absorption spectrophotometer and the flameless atomic absorption spectrophotometer: