Advanced editing of pathway genome databases
This presentation is the property of its rightful owner.
Sponsored Links
1 / 28

Advanced Editing of Pathway/Genome Databases PowerPoint PPT Presentation


  • 77 Views
  • Uploaded on
  • Presentation posted in: General

Advanced Editing of Pathway/Genome Databases. Ron Caspi. General Curation. User Preferences. Create and Use Author and Organization Frames. Using the Text Editor. Formatting <i> italic </i>, <b> bold </i> know your &alpha;, &beta; Text wrapper: need two newlines to force a new paragraph

Download Presentation

Advanced Editing of Pathway/Genome Databases

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Advanced editing of pathway genome databases

Advanced Editing of Pathway/Genome Databases

Ron Caspi


General curation

General Curation


User preferences

User Preferences


Create and use author and organization frames

Create and Use Author and Organization Frames


Using the text editor

Using the Text Editor

  • Formatting

    • <i>italic</i>, <b>bold</i>

    • know your &alpha;, &beta;

    • Text wrapper: need two newlines to force a new paragraph

    • Text wrapper: Never leave empty spaces at the end of a line

    • An internal link to a reaction frame will print the reaction equation

    • To print an enzymatic activity name use an internal link to the enzymatic activity frame ID, not the enzyme frame ID (important when an enzyme is multifunctional e.g. CPLX-6934).

    • When providing multiple citations, use |CITS:[PMID1][PMID2| (rather than |CITS:[PMID1]|, |CITS:[PMID2]|.

  • Special characters:

    • Ångstrom &Aring; (Å)

    • Degree &deg; (◦)


Use internal hyperlinks

Use Internal Hyperlinks


Use variant classes

Use Variant Classes

example: Putrescin Biosynthesis


Hypothetical reactions excluded enzymes

Hypothetical Reactions, Excluded Enzymes

Specified in the Pathway Info Editor

Enzymes Not Used : useful when an enzyme is associated with a reaction, but does not participate in a specific pathway. For example, a catabolic enzyme in a biosynthetic pathway (e.g. EC 2.1.3.3, ornithine carbamoyltransferase)

Hypothetical reactions: useful when a pathway step is proposed, but has not been proven


Super pathways

Super Pathways

  • Need to keep pathways within well-defined end points

  • Link pathways to upstream or downstream pathways with pathway links.

  • Keep pathways simple

  • Create more complex metabolic networks using superpathways

  • Example: superpathway of aromatic compound degradation (aerobic)

    is composed of:

  • catechol degradation II

  • mandelate degradation I

  • benzoate degradation (aerobic)

  • b-ketoadipate degradation

  • protocatechuate degradation II

  • shikimate degradation

  • quinate degradation

  • 4-hydroxymandelate degradation

  • tryptophan degradation I


Advanced curation

Advanced Curation


Using the frame editor

Using the Frame Editor

The frame editor is powerful, but dangerous… Use it when there are no alternatives.

Examples:

  • Renaming frames

  • Modified proteins

  • Modifying dates of author credits

  • Replacing an enzyme or reaction in an enzymatic-reaction frame

  • Removing mistakes from pathway frames, such as predecessor pairs that the software ignores.

  • Removing duplicated values from slots that should only have a single value (OFFIClAL-EC?)

  • Investigated orphan enzymatic reaction frames reported by the consistency Checker


Protein complexes

Protein complexes

Adenosylmethionine decarboxylase is first synthesized as a proenzyme, and then self-cleaves into two smaller polypeptides. Each cleavage product forms a homotetramer, and the two complexes form a heterooctamer.

A combination of editors enables creation of such multi-level complexes.

Tutorial: Creating Protein Complexes


Classes and instances

Classes and Instances

  • Instance framesdescribe specific objects (e.g. a specific gene)

  • Class framesdescribe general types of biological objects (e.g. the class of all genes)

  • Proteins that are substrates of MetaCyc reactions are classes

  • Every compound with an “R” in its structure should be a class


Converting an existing compound instance to a class

Converting an existing compound instance to a class

Modifications of MetaCyc classes is considered a schema change, and will be overwritten during the next update!

Only use this procedure to correct curation errors that were introduced in your PGDB!

  • Open the compound editor

  • Click “Convert to Class” and exit

  • Rename the frame to follow class name convention (if necessary)

  • Modify the common name to start with “a”


The ontology editor

The Ontology Editor


The ontology editor1

The Ontology Editor

  • Changing parent classes

  • Adding parent classes

  • Creating new classes to improve ontology

    Tutorial: the Ontology Editor


The consistency checker

The Consistency Checker

Consistency Checking should be performed routinely (every few months), and detected problems should be addressed


Consistency checker automatic tasks

Consistency Checker – Automatic Tasks

Bad Links

MetaCyc pathways are extensively linked to other pathways. When new PGDBs are created by Pathologic, these links are still there, even if they point to pathways that are not present in the new PGDB. These links are only removed by the Consistency Checker.


Consistency checker manual tasks

Consistency Checker – Manual Tasks

Example: create an empty |FRAME: | construct, then run the task “Check Frame References”


Exporting pathways between pgdbs

Exporting Pathways Between PGDBs

  • To export a pathway to a file:

    (optional inclusion of enzymes and genes)

    • Edit => Add Pathway to File Export List

    • File => Export => Selected Pathways to Lisp-format File

  • To import a pathway from file:

    • File => Import => Pathways from File

  • To export a pathway directly to another PGDB (both PGDBs must be installed on the same system):

    • Edit -> Export Pathway to DB


Moving objects between pgdbs

Moving Objects Between PGDBs

The following commands will import a frame from MetaCyc to EcoCyc:

Both databases must be open before this will work.

  • (import-compounds '(CPD-ID) (kb-of-organism 'meta) (kb-of-organism 'ecoli))

  • (import-reactions '(ID-RXN) (kb-of-organism 'meta) (kb-of-organism 'ecoli))

  • (import-proteins '(ID-MONOMER) (kb-of-organism 'meta) (kb-of-organism 'ecoli))


Exporting graphics

Exporting Graphics

  • You can save any screen as a vector-based postscript file by using File -> Print

  • The PS files are easily converted to PDF by Adobe Distiller (pat of the Acrobat Pro package)

  • Graphics programs like Corel Draw or Illustrator can open the PDF files and let you manipulate the graphics

  • The software also generates two posters – the cellular overview, and the genome poster. Those are also generated in postscript format.


Creating links to external databases

Creating Links to External Databases

  • To define a new external database link:

  • File → Create → External Database Description

  • Enter frame name

  • Fill fields as shown in next slide

  • To edit an existing link:

  • Right-click on a link (from a Navigator page), and select “Edit External Database Info”

  • Creating links to a PGDB

    see http://biocyc.org/linking.shtml


External database editor

External Database Editor


Polymerization

Polymerization

example: folate polyglutamylation I


The pathway registry

The Pathway Registry


The registry schema upgrades

The Registry – Schema Upgrades


The registry uploading your pgdb

The Registry – Uploading Your PGDB

The process of uploading a PGDB to the Registry is largely automated. See “Publishing PGDBs in the Registry” in the User Guide for details


  • Login